Recombinant Mouse Anti-VACV envelope protein D8 Antibody (LA5) (CAT#: PABZ-213)
Recombinant Mouse Antibody (LA5) is capable of binding to VACV envelope protein D8, expressed in Chinese Hamster Ovary cells (CHO). Monoclonal antibody LA5 binds to the VACV envelope protein D8, a 32-kDa type 1 membrane protein, binds to cell surface chondroitin sulfate (CS) but not to HS.
Specific Inquiry
Figure 2 D8 binds to CS-E and anti-D8 MAbs display different levels of competition with CS-E.
A. GAG microarray performed with monomeric D8 antigen. B. MAb/CS-E cross-blocking experiments using representatives of all four antibody specificity groups and oligomeric D8. C. MAb/CS-E cross-blocking of group III MAbs D. Summary of CS-E cross-blocking abilities of various MAbs. Group III MAbs are characterized by large variations in cross-blocking ability. Microarray binding experiments were performed in triplicate, and the data represent the average of 10 spots per concentration averaged from the three experiments (±SEM, error bars).
Matho, M. H., de Val, N., Miller, G. M., Brown, J., Schlossman, A., Meng, X., ... & Ward, A. B. (2014). Murine anti-vaccinia virus D8 antibodies target different epitopes and differ in their ability to block D8 binding to CS-E. PLoS pathogens, 10(12), e1004495.
Specifications
- Immunogen
- VACV IMV membrane protein D8
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native VACV envelope protein D8
- Species Reactivity
- VACV
- Clone
- LA5
- Applications
- ELISA 10⁻¹-10⁴ ng/mL
Block Assay-Dependent
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody LA5 has been reported in applications of ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
ELISA: A reported concentration of the antibody LA5 for ELISA was 10⁻¹-10⁴ ng/mL.
Target
- Alternative Names
- D8; IMV membrane protein
- UniProt ID
- Q1M1K6
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-213 | Recombinant Mouse Anti-VACV envelope protein D8 Antibody Fab Fragment (LA5) | ELISA, Neut | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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