Rabbit Anti-VHH Antibody

Customer Review

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Jessica Hill
22.Feb,23

Excellent for VHH Studies

The Anti-VHH Antibody has been excellent for our VHH studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

David Adams
29.May,22

Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for VHH is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Megan Martinez
13.Aug,21

Consistent High-Quality Results

This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for VHH have been particularly beneficial.

Q&As

1. Is the anti-VHH antibody suitable for use in Western blotting?

   A: Yes, the anti-VHH antibody (MOB-099CQ) is suitable for use in Western blotting. It provides specific binding to VHH, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-VHH antibody?

   A: The recommended storage condition for the anti-VHH antibody (MOB-099CQ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-VHH antibody be used in immunoprecipitation assays?

   A: Yes, the anti-VHH antibody (MOB-099CQ) can be used in immunoprecipitation assays. It provides specific binding to VHH, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-VHH antibody effective in ELISA applications?

   A: Yes, the anti-VHH antibody (MOB-099CQ) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of VHH.

5. What is the optimal dilution for using the anti-VHH antibody in immunofluorescence?

   A: The optimal dilution for using the anti-VHH antibody (MOB-099CQ) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Lamtha, Thomanai, et al. "Generation of a nanobody against HER2 tyrosine kinase using phage display library screening for HER2-positive breast cancer therapy development." Protein Engineering, Design and Selection 34 (2021): gzab030. https://doi.org/10.1093/protein/gzab030
   This study focuses on generating nanobodies targeting the HER2 tyrosine kinase domain, which is significant in HER2-positive breast cancer therapy. The research aimed to produce smaller, single-domain antibodies (nanobodies) to overcome limitations associated with conventional monoclonal antibodies, such as large size and resistance issues. Using phage display technology, the researchers identified three specific anti-HER2-TK nanobodies, with clone VHH17 showing the highest HER2 kinase inhibition activity. The study highlighted the potential of VHH17 as an effective therapeutic agent for treating HER2-positive breast cancer, demonstrating its ability to significantly decrease the viability of HER2-positive cancer cells.
   Creative Biolabs played a crucial role in this research by providing essential reagents. Specifically, they supplied the rabbit anti-VHH antibody (Cat# MOB-099CQ) used in the ELISA binding affinity determination experiments. This reagent was vital for detecting the binding interactions between the selected nanobodies and HER2-TK, enabling the researchers to assess the specificity and efficacy of the nanobodies. The involvement of Creative Biolabs' products facilitated the detailed characterization and validation of the nanobody VHH17, which contributed significantly to the advancement of this therapeutic strategy for HER2-positive breast cancer.
2. Damman, Reinier, et al. "Development of in vitro-grown spheroids as a 3D tumor model system for solid-state NMR spectroscopy." Journal of Biomolecular NMR 74.8 (2020): 401-412. https://doi.org/10.1007/s10858-020-00328-8
   This study explores the use of in vitro-grown spheroids as a three-dimensional tumor model system for solid-state NMR spectroscopy. Researchers investigated the hanging drop method to grow spheroids of uniform size and shape, suitable for studying solid tumors using solid-state NMR (ssNMR) spectroscopy. The findings revealed that these spheroids are stable under magic-angle-spinning conditions and exhibit significant metabolic differences compared to single cell preparations. Dynamic nuclear polarization (DNP)-supported ssNMR measurements demonstrated that even low concentrations of labeled nanobodies targeting EGFR (7D12) could be detected inside the spheroids. These results suggest that solid-state NMR can effectively examine proteins or other biomolecules within a 3D cellular microenvironment, offering potential applications in pharmacological research.
   Creative Biolabs contributed significantly to this research by providing the rabbit-α-VHH antibody (Cat# MOB-099CQ) used for detecting 7D12. This antibody was crucial for the nanobody binding assay, which involved the detection of 7D12 binding to EGFR on A431 cells. The presence of this antibody enabled precise measurement of fluorescence intensity, helping to quantify the binding curve of 7D12. This contribution was essential for verifying the functionality and penetration of 7D12 into the spheroids, thereby facilitating detailed structural and functional studies of the proteins within the tumor model system.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-099CQ, RRID: AB_3111481)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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