Human IgG4 Isotype Control Antibody (CAT#: MOB-0408ZL)
Recombinant Human IgG4 monoclonal antibody specifically binds to a hapten that is not expressed in mammalian cells.
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SDS-PAGE
Figure 1 Human IgG4 Isotype Control Antibody (MOB-0408ZL) in SDS-PAGE
SDS-PAGE analysis of MOB-0408ZL in reduced (lane 1) and non-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of MOB-0408ZL was greater than 95% as determined by SDS-PAGE.
Specifications
- Immunogen
- Hapten molecule not expressed in mammalian cells
- Host Species
- Human
- Type
- Human IgG4, κ
- Clone
- FU015
- Applications
- ELISA, IB, FC, IHC, FuncS
Product Property
- Purification
- Affinity chromatography purified.
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IGHG4; immunoglobulin heavy chain constant region gamma 4
Related Resources
Choi, Seong Jin, et al. "KIR+ CD8+ and NKG2A+ CD8+ T cells are distinct innate-like populations in humans." Cell Reports 42.3 (2023). https://doi.org/10.1016/j.celrep.2023.112236
This study explores the heterogeneity among human KIR+CD8+ and NKG2A+CD8+ T cells, identifying them as distinct innate-like populations with different cytokine responsiveness. The research demonstrates that KIR+CD8+ T cells exhibit IL-15-induced NK-like cytotoxicity, while NKG2A+CD8+ T cells show IL-12/IL-18-induced IFN-gamma production. It was observed that KIR+CD8+ T cells are more terminally differentiated and replicative senescent compared to NKG2A+CD8+ T cells, which are more responsive to IL-12 and IL-18 due to the high expression of these receptors induced by the transcription factor PLZF.
At Creative Biolabs, we provided essential reagents for this study, including the anti-NKG2A antibody (Cat#: TAB-437CQ) and the IgG4 isotype control (Cat# MOB-0408ZL) used in the flow cytometry analyses. Our products were crucial in identifying the distinct functional roles and cytokine responses of these T cell subsets. This research underscores the importance of understanding the diverse functions of T cell populations in developing targeted immunotherapies, highlighting our commitment to advancing immunological research and innovative therapeutic solutions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
Customer Review
Q&As
David Walker
Excellent for IgG4 Control
Excellent for IgG4 Control
The Human IgG4 Isotype Control Antibody has been excellent for our IgG4 control studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
09-Sep-23
Emily Turner
Reliable in Immunoassays
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for IgG4 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
11-Oct-22
John Harris
Consistent and High-Quality
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for IgG4 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
20-Jul-21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-0408ZL, RRID: AB_3111477)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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