What research applications is Selicrelumab suitable for?

Selicrelumab is primarily used in applications such as ELISA, immunohistochemistry (IHC), flow cytometry (FC), and immunoprecipitation (IP). These methods help in analyzing the expression of CD40 in various samples and understanding its implications in solid tumors.

What diseases can Selicrelumab help study?

This antibody is particularly useful in studying solid tumors. Its ability to target CD40 makes it a valuable tool in cancer research, providing insights into immune response modulation and tumor progression.

Is this antibody available in a conjugated form?

While the standard offering is unconjugated, there are conjugation options available, such as PE, APC, Biotin, and FITC. These can be selected according to the specific needs of the research being conducted.

Anti-Human CD40 Recombinant Antibody (Selicrelumab)

CAT#: TAB-470CQ

Recombinant monoclonal antibody to CD40. Selicrelumab is a human monoclonal antibody that can be potentially used in the treatment of Solid tumors.

Gene Expression Tested Data

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72868/156401_A_8_8.jpg

Normal Tissue Figure 2 Tonsil

Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31567/156626_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, EFO-21)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101017-CD40

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SDS-PAGE

Figure 1 Anti-Human CD40 Recombinant Antibody (Selicrelumab) (TAB-470CQ) in SDS-PAGE

SDS-PAGE analysis of TAB-470CQ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-470CQ was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 2 Anti-Human CD40 Recombinant Antibody (Selicrelumab) (TAB-470CQ) in HPLC

HPLC analysis of TAB-470CQ shows the purity is>95%.

Figure 3 Anti-Human CD40 Recombinant Antibody (Selicrelumab) (TAB-470CQ) in WB

Western blot analysis of TAB-470CQ was performed by loading human CD40.
TAB-470CQ incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane1: Reducing antigen (0.1μg)
Lane2: Reducing antigen (0.3μg)
Lane3: Reducing antigen (0.6μg)

ELISA

Figure 4 Anti-Human CD40 Recombinant Antibody (Selicrelumab) (TAB-470CQ) in ELISA

ELISA analysis of TAB-470CQ was performed by coating with human CD40.
The secondary antibody: HRP-Anti-Human IgG (H+L)

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG2, κ

Specificity
Human CD40

Species Reactivity
Human

Applications
ELISA, IHC, FC, IP, IF, FuncS

Conjugate
Unconjugated

CAS
1622140-49-1

Generic Name
Selicrelumab

UNII
0O39RGI33V

Related Disease
Solid tumors

Product Property

Purity
> 95% as determined by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The CD40 antibody has been reported in applications of PK.
Pharmacokinetic: Lucatumumab serum levels were collected before dosing, at the end of the infusion, 2 h after the end of the infusion and 24, 48, 96 and 168 h after the start of the first and fourth infusions. Additional time points beyond 168 h were collected after the fourth infusion at 336, 504, 672 and 840 h and every other week thereafter as well as before and after the second and third infusions.

Target

Alternative Names
p50; Bp50; CDW40; TNFRSF5

Gene ID
958

UniProt ID
P25942

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Breast cancer biomarkers at key points during disease progression

Highly Reliable in Cancer Research

Selicrelumab has proven incredibly useful in our studies on solid tumors. Its high specificity and sensitivity to CD40 allow us to effectively monitor and analyze tumor-immune interactions. This antibody has significantly contributed to the reliability and depth of our cancer biomarker research, enabling us to draw insightful conclusions.

Excellent for Flow Cytometry

Utilizing Selicrelumab in flow cytometry has been straightforward and effective. Its high affinity and precise targeting capabilities have allowed us to accurately assess CD40 expression, which is key in our research on tumor microenvironments. We appreciate its stability and consistency across experiments.

A Robust Tool for Immunoprecipitation

Our lab consistently sees positive outcomes with Selicrelumab in immunoprecipitation applications. Its robustness and purity ensure we can isolate CD40 with minimal background interference, providing clean results necessary for analyzing protein interactions in solid tumors.

Q&As

What research applications is Selicrelumab suitable for?

A: Selicrelumab is primarily used in applications such as ELISA, immunohistochemistry (IHC), flow cytometry (FC), and immunoprecipitation (IP). These methods help in analyzing the expression of CD40 in various samples and understanding its implications in solid tumors.
What diseases can Selicrelumab help study?

A: This antibody is particularly useful in studying solid tumors. Its ability to target CD40 makes it a valuable tool in cancer research, providing insights into immune response modulation and tumor progression.
Is this antibody available in a conjugated form?

A: While the standard offering is unconjugated, there are conjugation options available, such as PE, APC, Biotin, and FITC. These can be selected according to the specific needs of the research being conducted.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-470CQ, RRID: AB_3111960)

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Related Diseases

Asthma

Systemic Lupus Erythematosus

Primary Immunodeficiency

Allograft Rejection

Autoimmune Thyroid Disease

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-470CQ.pdf

MSDS

MSDS - TAB-470CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Selicrelumab"

Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-470CQ)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to CD40. It is a human monoclonal antibody that can be potentially used in the treatment of Solid tumors.

See other products for "CD40"

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AGTO-G079E	Anti-CD40 immunotoxin (scFv)-PE	Cytotoxicity assay, Function study

CAT	Product Name	Application
TAB-0063CL-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (PPAT-071-03)	ELISA, FuncS
TAB-0064CL-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (PPAT-071-04)	ELISA, FuncS
TAB-0065CL-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (PPAT-071-05)	ELISA, FuncS
TAB-0066CL-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (PPAT-071-06)	ELISA, FuncS
TAB-0067CL-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (PPAT-071-07)	ELISA, FuncS

CAT	Product Name	Application	Type
TAB-188LC	Anti-Human CD40 Recombinant Antibody (chi220)	SPR, ELISA, Inhib, FuncS	Chimeric antibody (mouse/human)
TAB-202LC	Anti-CD40 Recombinant Antibody (TAB-202LC)	ELISA, FC, FuncS	Chimeric (mouse/rhesus) IgG1
TAB-203LC	Anti-CD40 Recombinant Antibody (TAB-203LC)	ELISA, FC, FuncS	Chimeric (mouse/rhesus) IgG4
TAB-188LC-S(P)	Anti-Human CD40 Recombinant Antibody scFv Fragment (chi220)	SPR, ELISA	Chimeric antibody (mouse/human)
TAB-192LC-S(P)	Mouse Anti-CD40 Recombinant Antibody; scFv Fragment (TAB-192LC-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
TAB-195LC	Human Anti-CD40 Recombinant Antibody (TAB-195LC)	ELISA, Agonist	Human IgG2
TAB-196LC	Human Anti-CD40 Recombinant Antibody (TAB-196LC)	ELISA, Agonist	Human IgG2
TAB-197LC	Human Anti-CD40 Recombinant Antibody (TAB-197LC)	ELISA, Agonist	Human IgG2
TAB-198LC	Human Anti-CD40 Recombinant Antibody (TAB-198LC)	ELISA	Human IgG2
TAB-006ML	Anti-Human CD40 Recombinant Antibody (Bleselumab)	ELISA, IHC, FC, IP, IF, Inhib	IgG4, κ

CAT	Product Name	Application	Type
TAB-189LC-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (F4)	SPR, ELISA	Humanized antibody
TAB-190LC-F(E)	Anti-Human CD40 Recombinant Antibody Fab Fragment (L3.17)	SPR, ELISA	Humanized antibody
TAB-194LC-F(E)	Human Anti-CD40 Recombinant Antibody; Fab Fragment (TAB-194LC-F(E))	ELISA	Humanized Fab
TAB-200LC-F(E)	Human Anti-CD40 Recombinant Antibody; Fab Fragment (TAB-200LC-F(E))	ELISA	Human Fab
TAB-204LC-F(E)	Human Anti-CD40 Recombinant Antibody; Fab Fragment (TAB-204LC-F(E))	SPR, ELISA	Human Fab

CAT	Product Name	Application	Type
NEUT-362CQ	Human Anti-CD40 Recombinant Antibody (clone 5D12)	Block, ELISA, FC, SPR, IP, IHC	Human IgG4, κ
NEUT-363CQ	Mouse Anti-CD40 Recombinant Antibody (clone 5C3)	FC, Block, Activ	Mouse IgG1, κ
NEUT-366CQ	Rat Anti-CD40 Recombinant Antibody (clone MAB0819)	Block, Activ	Rat IgG2

CAT	Product Name	Application	Type
NEUT-364CQ	Mouse Anti-CD40 Recombinant Antibody (clone CBL829)	WB, Neut	Mouse IgG2b, κ
NEUT-367CQ	Mouse Anti-CD40 Recombinant Antibody (clone CBL013)	WB, IHC, Neut	Mouse IgG2

CAT	Product Name	Application	Type
NEUT-365CQ	Hamster Anti-CD40 Recombinant Antibody (clone HM40-3)	FC, Block, Costim, IHC	Hamster IgM

CAT	Product Name	Application	Type
MOR-4059	Hi-Affi™ Rabbit Anti-CD40 Recombinant Antibody (clone SI126DS)	ELISA	Rabbit IgG

CAT	Product Name	Application	Type
MOB-105LC	Human Anti-CD40 Recombinant Antibody (MOB-105LC)	FC, FuncS	Human IgG1, κ
VS-0724-XY18	Mouse Anti-CD40 Agonistic Antibody (VS-0724-XY18)	FC, Agonistic assays, CyTOF®, ICC	Mouse IgG2b
VS-0724-XY19	Rat Anti-Cd40 Agonistic Antibody (VS-0724-XY19)	FC, IP, Agonistic assays, CyTOF®	Rat IgG2a
VS-0724-XY20	Rabbit Anti-Cd40 Agonistic Antibody (VS-0724-XY20)	FC, WB, FuncS, Activ, Agonistic assays	Rabbit IgG, kappa
VS-0724-XY21	Rat Anti-Cd40 Agonistic Antibody (clone FGK45)	FC, WB, FuncS, Activ, Agonistic assays	Rat IgG2a, kappa

CAT	Product Name	Application	Type
HPAB-0162-YC-S(P)	Human Anti-CD40 Recombinant Antibody; scFv Fragment (HPAB-0162-YC-S(P))	FC	Human scFv
HPAB-0163-YC-S(P)	Human Anti-CD40 Recombinant Antibody; scFv Fragment (HPAB-0163-YC-S(P))	FC	Human scFv
HPAB-0164-YC-S(P)	Human Anti-CD40 Recombinant Antibody; scFv Fragment (HPAB-0164-YC-S(P))	FC, Agonist	Human scFv
HPAB-0165-YC-S(P)	Human Anti-CD40 Recombinant Antibody; scFv Fragment (HPAB-0165-YC-S(P))	FC	Human scFv
HPAB-0166-YC-S(P)	Human Anti-CD40 Recombinant Antibody (clone S2C6); scFv Fragment	FC, FuncS	Human scFv

CAT	Product Name	Application	Type
NS-021CN	Human Anti-CD40 Recombinant Antibody (NS-021CN)	ELISA, IF, Block, FuncS	Human IgG
NS-021CN-F(E)	Human Anti-CD40 Recombinant Antibody; Fab Fragment (NS-021CN-F(E))	ELISA	Human Fab
NS-021CN-S(P)	Human Anti-CD40 Recombinant Antibody; scFv Fragment (NS-021CN-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0009-WJ-F(E)	Human Anti-CD40 Recombinant Antibody; Fab Fragment (HPAB-0009-WJ-F(E))	ELISA	Human Fab
HPAB-1662LY-F(E)	Mouse Anti-CD40 Recombinant Antibody; Fab Fragment (HPAB-1662LY-F(E))	ELISA, RIA	Mouse Fab
HPAB-1663LY-F(E)	Mouse Anti-CD40 Recombinant Antibody; Fab Fragment (HPAB-1663LY-F(E))	ELISA, RIA	Mouse Fab
HPAB-1664LY-F(E)	Mouse Anti-CD40 Recombinant Antibody; Fab Fragment (HPAB-1664LY-F(E))	ELISA, RIA	Mouse Fab
HPAB-1665LY-F(E)	Mouse Anti-CD40 Recombinant Antibody; Fab Fragment (HPAB-1665LY-F(E))	ELISA, RIA	Mouse Fab

CAT	Product Name	Application	Type
PABC-256	Mouse Anti-CD40 Recombinant Antibody (PABC-256)	ELISA, FC, WB, Agonist	Mouse IgG
HPAB-M0481-YC	Human Anti-CD40 Recombinant Antibody (HPAB-M0481-YC)	ELISA, FC	Human IgG
HPAB-M0482-YC	Human Anti-CD40 Recombinant Antibody (HPAB-M0482-YC)	ELISA, FC	Human IgG
HPAB-M0483-YC	Human Anti-CD40 Recombinant Antibody (HPAB-M0483-YC)	ELISA, FC	Human IgG
HPAB-M0484-YC	Human Anti-CD40 Recombinant Antibody (HPAB-M0484-YC)	ELISA, FC	Human IgG

CAT	Product Name	Application	Type
AFC-TAB-152	Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-152)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-262	Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-262)	IP, IF, FuncS, FC, Neut, ELISA	ADCC enhanced antibody
AFC-TAB-759	Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-759)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-470CQ	Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-470CQ)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-006ML	Afuco™ Anti-CD40 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-006ML)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-1218WJ	Recombinant Llama Anti-CD40 Single Domain Antibody (HPAB-1218WJ)	ELISA	Llama VHH
HPAB-1219WJ	Recombinant Llama Anti-CD40 Single Domain Antibody (HPAB-1219WJ)	ELISA	Llama VHH
HPAB-1220WJ	Recombinant Llama Anti-CD40 Single Domain Antibody (HPAB-1220WJ)	ELISA	Llama VHH

CAT	Product Name	Application	Type
VS-0724-YC1328	AbPlus™ Anti-CD40 Magnetic Beads (VS-0724-YC1328)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC117	Mouse Anti-CD40 Recombinant Antibody Hexamer (VS-0924-YC117), CDC Enhanced	ELISA, ADCC, CDC	Antibody hexamer
VS-0924-YC118	Human Anti-CD40 Recombinant Antibody Hexamer (VS-0924-YC118), CDC Enhanced	ELISA, Block, FC, IF, Activ, CDC	Antibody hexamer

CAT	Product Name	Application	Type
VS-0225-XY52	CytoStream™ Armenian Hamster Anti-CD40 Recombinant Antibody (clone HM40-3)	FC, Block	Armenian Hamster IgM, kappa

CAT	Product Name	Application	Type
VS-0325-FY89	Rat Anti-CD40 (clone FGK45) scFv-Fc Chimera	FC, WB, Agonist	Rat IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC514	Recombinant Anti-CD40 Vesicular Antibody, EV Displayed (VS-0425-YC514)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1181	Anti-CD40 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC30	Recombinant Anti-CD40 (CRD1 x ECD) Biparatopic Antibody, Tandem scFv	ELISA, FC, Neut, IHC, IP, IF	Tandem scFv
VS-0525-YC206	Recombinant Anti-CD40 (CRD2 x CRD1) Biparatopic Antibody, Tandem scFv	ELISA, FC, IF, IHC, IP	Tandem scFv
VS-0525-YC208	Recombinant Anti-CD40 Biparatopic Antibody, Tandem scFv (Bleselumab x Selicrelumab)	ELISA, FC, IP, IF, IHC	Tandem scFv

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