Mouse Anti-2-AAF Recombinant Antibody (clone K16-16) (CAT#: FAMAB-1307CQ)

This product is a recombinant mouse antibody that recognizes 2-AAF. The antibody was purified by affinity chromatography. It can be used in DB hydridization, PCR, SB.

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ELISA

Figure 1 Binding specificity of the recombinant scFv–PhoAv conjugate determined by ELISA.

Figure 1 Binding specificity of the recombinant scFv–PhoAv conjugate determined by ELISA.

Microtiter plates were coated with unlabeled (▲) or AAF-labeled (●) SSS-DNA. (A) Conventional two-step procedure using the anti-AAF monoclonal antibody. Absorbance values were read after 10 min enzyme reaction. (B) One-step procedure using the anti-AAF scFv-PhoAv conjugate. Absorbance values were read after 20 min enzyme reaction. The coefficient of variation ranged from 1.5% to 5.5% for the Mab and from 0.3% to 2.4% for the scFv-PhoAv conjugate.

Mueller, G. A., Ankney, J. A., Glesner, J., Khurana, T., Edwards, L. L., Pedersen, L. C., ... & London, R. E. (2014). Characterization of an anti-Bla g 1 scFv: epitope mapping and cross-reactivity. Molecular immunology, 59(2), 200-207.


Specifications

  • Immunogen
  • Acetyl aminofluorene-substituted guanosine
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • AAF-labelled DNA
  • Clone
  • K16-16
  • Applications
  • DB hydridization, PCR, SB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Acetylaminofluorene; AAF; 2-AAF

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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