Rabbit Anti-AK4 Recombinant Antibody (clone R08-2B2) (CAT#: VS3-FY1966)
This purified recombinant primary antibody is capable of binding to human AK4. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-AK4 Antibody (VS3-FY1966) in ICC.
Immunocytochemistry staining of AK3L1 (green) in Hela using the anti-AK3L1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Figure 2 Anti-AK4 Antibody (VS3-FY1966) in WB.
The AK3L1 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-AK3L1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptides of human AK3L1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AK4
- Species Reactivity
- Human
- Clone
- R08-2B2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Reticular Dysgenesis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Adenylate Kinase 4; Adenylate Kinase 3-Like 1; AK3L1; AK 4; AK3; GTP:AMP Phosphotransferase AK4, Mitochondrial; Adenylate Kinase Isoenzyme 4, Mitochondrial; Nucleoside-Triphosphate-Adenylate Kinase; Adenylate Kinase 4, Mitochondrial; Mitochondrial Adenylate Kinase-3
- Gene ID
- 205
- UniProt ID
- P27144
- Sequence Similarities
- Belongs to the adenylate kinase family. AK3 subfamily.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P27144)
Modification sites at neXtProt (NX_P27144)
- Protein Refseq
- XP_016856102.1; XP_047305130.1; XP_054191032.1; XP_054191033.1; NP_001005353.1
- Function
- Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK.
Plays a protective role in the cellular response to oxidative stress.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "AK4"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2946z | Mouse Anti-AK4 Recombinant Antibody (clone 28G11) | WB, FC, IF, IHC | Mouse IgG1 |
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