Rabbit Anti-AK4 Recombinant Antibody (clone R08-2B2) (CAT#: VS3-FY1966)
This purified recombinant primary antibody is capable of binding to human AK4. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We confirm the QC result of the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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ICC
Figure 1 Anti-AK4 Antibody (VS3-FY1966) in ICC.
Immunocytochemistry staining of AK3L1 (green) in Hela using the anti-AK3L1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-AK4 Antibody (VS3-FY1966) in WB.
The AK3L1 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-AK3L1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptides of human AK3L1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AK4
- Species Reactivity
- Human
- Clone
- R08-2B2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 25 kDa, while the observed molecular weight stands at 25 kDa.
- Related Disease
- Reticular Dysgenesis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Adenylate Kinase 4; Adenylate Kinase 3-Like 1; AK3L1; AK 4; AK3; GTP:AMP Phosphotransferase AK4, Mitochondrial; Adenylate Kinase Isoenzyme 4, Mitochondrial; Nucleoside-Triphosphate-Adenylate Kinase; Adenylate Kinase 4, Mitochondrial; Mitochondrial Adenylate Kinase-3
- Gene ID
- 205
- UniProt ID
- P27144
- Sequence Similarities
- Belongs to the adenylate kinase family. AK3 subfamily.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P27144)
Modification sites at neXtProt (NX_P27144)
- Protein Refseq
- XP_016856102.1; XP_047305130.1; XP_054191032.1; XP_054191033.1; NP_001005353.1
- Function
- Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK.
Plays a protective role in the cellular response to oxidative stress.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2946z | Mouse Anti-AK4 Recombinant Antibody (clone 28G11) | WB, FC, IF, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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