Rabbit Anti-B7-H2 Recombinant Antibody (VS-0723-WK198) (CAT#: VS-0723-WK198)
This product is a rabbit IgG antibody that recognizes human B7-H2, which is the ligand for the T-cell-specific cell surface receptor ICOS. This antibody is recommended for flow cytometry (1/100) and ELISA (1/5000-1/10000) assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane & cytoplasmic bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29179/1168_B11_4_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane & cytoplasmic bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29179/1168_B11_4_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_8_selected.jpg
Figure 3 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79997/170635_B_6_4_selected.jpg
(Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79997/170635_B_6_4_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_B_9_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_8_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_8_4.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_5_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26037/59019_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (Daudi)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160223-ICOSLG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160223-ICOSLG
Specifications
- Immunogen
- Recombinant human B7-H2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human B7-H2
- Species Reactivity
- Human
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 1×PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/5000-1/10000
Flow Cytometry: 1/100
Target
- Alternative Names
- ICOSLG; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; ICOS ligand
- Gene ID
- 23308
- UniProt ID
- O75144
- Long Name
- Inducible T Cell Costimulator Ligand
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1141CL | Anti-Human B7-H2 Recombinant Antibody (20B10) | FC, WB, DB, ELISA | |
| TAB-1141CL-S(P) | Anti-Human B7-H2 Recombinant Antibody scFv Fragment (20B10) | FC, WB, DB, ELISA | |
| TAB-1141CL-F(E) | Anti-Human B7-H2 Recombinant Antibody Fab Fragment (20B10) | FC, WB, DB, ELISA |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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