Provided are human antibodies that bind to the BoNT/A (botulinum neurotoxin serotype A) binding domain HC and exhibited neutralizing activity in the mouse hemidiaphragm assay.
Figure 1 Human Anti-BoNT/A HC Antibody (3D12) in Neut.
In vitro toxin neutralization by mAb, pairs of mAbs, and oligoclonal Ab. Time to 50% twitch reduction was measured in isolated mouse hemidiaphragms and reported for toxin only control, single mAb (C25, S25, or 3D12), pairs of mAbs (C25+S25, C25+3D12, or 3D12+S25), and oligoclonal Ab (C25+3D12, +S25). Single mAb significantly prolonged time to neuroparalysis compared with toxin only. Pairs of mAbs significantly prolonged time to neuroparalysis compared with single mAbs.
Nowakowski, A., Wang, C., Powers, D. B., Amersdorfer, P., Smith, T. J., Montgomery, V. A., ... & Marks, J. D. (2002). Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proceedings of the National Academy of Sciences, 99(17), 11346-11350.
Figure 2 Human Anti-BoNT/A HC Antibody (3D12) in Neut.
In vivo toxin neutralization by mAbs and pairs of mAbs. Fifty micrograms total Ab was mixed with 20 or 100 mouse LD50s of toxin and injected i.p. Time to death and number of surviving mice was determined. No single mAb showed significant protection against 20 LD50s. All mice survived challenge with 100 LD50s when given any pair of mAbs.
Nowakowski, A., Wang, C., Powers, D. B., Amersdorfer, P., Smith, T. J., Montgomery, V. A., ... & Marks, J. D. (2002). Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proceedings of the National Academy of Sciences, 99(17), 11346-11350.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0034YC | Recombinant Human Anti-BoNT/A HC Antibody (3F10) | ELISA, Neut | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0033-YC-S(P) | Recombinant Human Anti-BoNT/A HC Antibody scFv Fragment (3D12) | ELISA, Neut | Human scFv |
FAMAB-0034-YC-S(P) | Recombinant Human Anti-BoNT/A HC Antibody scFv Fragment (3F10) | ELISA, Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0033-YC-F(E) | Recombinant Human Anti-BoNT/A HC Antibody Fab Fragment (3D12) | ELISA, Neut | Human Fab |
FAMAB-0034-YC-F(E) | Recombinant Human Anti-BoNT/A HC Antibody Fab Fragment (3F10) | ELISA, Neut | Human Fab |
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