Recombinant Human Anti-BoNT/A HC Antibody (3D12) (CAT#: FAMAB-0033YC)
Provided are human antibodies that bind to the BoNT/A (botulinum neurotoxin serotype A) binding domain HC and exhibited neutralizing activity in the mouse hemidiaphragm assay.
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Custom Production
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Neut
Figure 1 Human Anti-BoNT/A HC Antibody (3D12) in Neut.
In vitro toxin neutralization by mAb, pairs of mAbs, and oligoclonal Ab. Time to 50% twitch reduction was measured in isolated mouse hemidiaphragms and reported for toxin only control, single mAb (C25, S25, or 3D12), pairs of mAbs (C25+S25, C25+3D12, or 3D12+S25), and oligoclonal Ab (C25+3D12, +S25). Single mAb significantly prolonged time to neuroparalysis compared with toxin only. Pairs of mAbs significantly prolonged time to neuroparalysis compared with single mAbs.
Nowakowski, A., Wang, C., Powers, D. B., Amersdorfer, P., Smith, T. J., Montgomery, V. A., ... & Marks, J. D. (2002). Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proceedings of the National Academy of Sciences, 99(17), 11346-11350.
Neut
Figure 2 Human Anti-BoNT/A HC Antibody (3D12) in Neut.
In vivo toxin neutralization by mAbs and pairs of mAbs. Fifty micrograms total Ab was mixed with 20 or 100 mouse LD50s of toxin and injected i.p. Time to death and number of surviving mice was determined. No single mAb showed significant protection against 20 LD50s. All mice survived challenge with 100 LD50s when given any pair of mAbs.
Nowakowski, A., Wang, C., Powers, D. B., Amersdorfer, P., Smith, T. J., Montgomery, V. A., ... & Marks, J. D. (2002). Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proceedings of the National Academy of Sciences, 99(17), 11346-11350.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- BoNT/A HC
- Species Reactivity
- BoNT/A
- Clone
- 3D12
- Applications
- ELISA, Neutralization
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- HC; Botulinum neurotoxin binding domain
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0034YC | Recombinant Human Anti-BoNT/A HC Antibody (3F10) | ELISA, Neut | Human IgG |
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