Recombinant Mouse Anti-BsRNaseY Antibody (RY79-90) (CAT#: PABC-528)
This product is a recombinant Mouse antibody that can recognize BsRNaseY. The antibody was raised against a 12-residue conserved peptide from the N-terminal noncatalytic domain of B. subtilis RNase Y (BsRNaseY) that is predicted to be intrinsically disordered.
Specific Inquiry
WB
Figure 1 Mouse Anti-BsRNaseY Antibody (RY79-90) in WB.
In vitro formation of the BsRNaseY/Fab RY79-90 complex. BsRNaseY was incubated with Fab RY79-90 at different ratios and loaded on a 4% native gel for gel shift assay. 1: Fab alone (2 μg); 2: BsRNaseY alone (2 μg); 3 to 13: BsRNaseY (5 μM) was mixed with 0.1 (3), 0.5 (4), 1 (5), 1.5 (6), 2 (7), 2.5 (8), 3 (9), 3.5 (10), 4 (11), 4.5 (12), and 5 μM (13) Fab RY79-90.
Hardouin, P. , Velours, C. , Bou-Nader, C. , Assrir, N. , & Golinelli-Pimpaneau, B. . (2018). Dissociation of the dimer of the intrinsically disordered domain of rnase y upon antibody binding. Biophysical Journal, 115(11).
SEC-MALS
Figure 2 Mouse Anti-BsRNaseY Antibody (RY79-90) in SEC-MALS.
SEC-MALS analysis of Nter-BsRNaseY and the Nter-BsRNaseY/Fab RY79-90 complex. Determination of the dissociation constant of the Nter-BsRNaseY homodimer. Nter-BsRNaseY was loaded at various concentrations (30-945 μM) onto a Superdex 200 10/300 GL increase column equilibrated with 40 mM MES (pH 6.8), 200 mM NaCl.
Hardouin, P. , Velours, C. , Bou-Nader, C. , Assrir, N. , & Golinelli-Pimpaneau, B. . (2018). Dissociation of the dimer of the intrinsically disordered domain of rnase y upon antibody binding. Biophysical Journal, 115(11).
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Bacillus subtilis RNase Y
- Species Reactivity
- B. subtilis (strain 168)
- Clone
- RY79-90
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- Bacillus subtilis RNase Y; BsRNaseY; rny
- UniProt ID
- O31774
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-528 | Recombinant Mouse Anti-BsRNaseY Antibody Fab Fragment (RY79-90) | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-528 | Recombinant Mouse Anti-BsRNaseY Antibody scFv Fragment (RY79-90) | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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