Recombinant Mouse Anti-C. botulinum HA70 Antibody (NAP83-27-7-6) (CAT#: FAMAB-0038CQ)

Recombinant Mouse Antibody clone NAP83-27-7-6, which is specific to C. botulinum HA70. This antibody can bind HA70 protein both in solution under physiological conditions and when immobilized in direct binding ELISA.


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ELISA

Figure 1 Antibody binding to recombinant hemagglutinin-GST fusion peptides of hemagglutinins by ELISA.

Figure 1 Antibody binding to recombinant hemagglutinin-GST fusion peptides of hemagglutinins by ELISA.

Microtiter plates were coated with 5 μg/ml of the hemagglutin-GST fusion peptide of BoNT/A complex (A-HA17, A-HA34, A-HA70) and BoNT/B complex (B-HA17, B-HA34, B-HA70). Antibodies are represented as follows: NAP80-5, blue bar; NAP80-7, magenta bar; NAP80-33, yellow bar; NAP83-27, light green bar; NAP83-71, orange bar; NAP83-97, gray bar.

Scotcher, M. C., Cheng, L. W., Ching, K., McGarvey, J., Hnasko, R., & Stanker, L. (2013). Development and characterization of six monoclonal antibodies to hemagglutinin-70 of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal antibodies in immunodiagnosis and immunotherapy, 32(1), 6-15.

WB

Figure 2 Antibody binding on Western blots to BoNT/A and BoNT/B toxin complex component proteins.

Figure 2 Antibody binding on Western blots to BoNT/A and BoNT/B toxin complex component proteins.

900 kDa BoNT/A complex (even numbered lanes), and BoNT/B complex (odd numbered lanes) separated on 10% SDS-PAGE in the presence of 10 mM DTT, transferred to nitrocellulose membranes and probed with MAbs NAP80-5 (lanes 3,4), NAP80-7 (lanes 5,6), NAP80-33 (lanes 7,8), NAP83-27 (lanes 9,10), NAP83-71 (lanes 11,12), and NAP83-97 (lanes 13-14). Lanes 1,2 show the serotype A and B complex components stained with SilverXpress Silver Stain; lanes15,16 show biotinylated BoNT/A and BoNT/B toxin complexes probed with 125 ng/ml Streptavidin-HRP conjugate in TBST (Zymed). Protein relative molecular weight (rMW) is indicated in kDa. Holo, 150 kDa holotoxin; NTNH, 120 kDa non-toxin non-hemagglutinin; N-106, 106 kDa processed fragment of NTNH; Hc, 100 kDa toxin heavy chain; Lc, 50 kDa toxin light chain; HA52, 52 kDa processed fragment of 70 kDa hemagglutinin; HA34, 34 kDa hemagglutinin; HA20, 20 kDa processed fragment of 70 kDa hemagglutinin; HA17, 17 kDa hemagglutinin; N-14, 14 kDa processed fragment of NTNH.

Scotcher, M. C., Cheng, L. W., Ching, K., McGarvey, J., Hnasko, R., & Stanker, L. (2013). Development and characterization of six monoclonal antibodies to hemagglutinin-70 of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal antibodies in immunodiagnosis and immunotherapy, 32(1), 6-15.

WB

Figure 3 Western blot analysis of PTC, serotype A (lanes 1,3,5) and B (lanes 2,4,6) separated by native polyacrylamide gel electrophoresis.

Figure 3 Western blot analysis of PTC, serotype A (lanes 1,3,5) and B (lanes 2,4,6) separated by native polyacrylamide gel electrophoresis.

Blots were probed with MAbs NAP80-33 (lanes 1,2) and NAP83-27 (lanes 3,4). Lanes 5,6 represent PTC serotype A and B, respectively, stained with Coomassie.

Scotcher, M. C., Cheng, L. W., Ching, K., McGarvey, J., Hnasko, R., & Stanker, L. (2013). Development and characterization of six monoclonal antibodies to hemagglutinin-70 of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal antibodies in immunodiagnosis and immunotherapy, 32(1), 6-15.

ELISA

Figure 4 Antibody binding to BoNT/A and BoNT/B 900 kDa neurotoxin complexes by direct binding ELISA.

Figure 4 Antibody binding to BoNT/A and BoNT/B 900 kDa neurotoxin complexes by direct binding ELISA.

Microtiter plates coated with 100 mL of a 300 ng/ml solution of BoNT/A toxin complex (♦) or BoNT/Btoxin complex (□). Data represent the average of three replicates – one standard error.

Scotcher, M. C., Cheng, L. W., Ching, K., McGarvey, J., Hnasko, R., & Stanker, L. (2013). Development and characterization of six monoclonal antibodies to hemagglutinin-70 of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal antibodies in immunodiagnosis and immunotherapy, 32(1), 6-15.

Figure 5 Capture of biotin-labeled 900 kDa BoNT/A and BoNT/B toxin complexes by anti-HA70 MAbs.

Figure 5 Capture of biotin-labeled 900 kDa BoNT/A and BoNT/B toxin complexes by anti-HA70 MAbs.

Biotinylated BoNT/A toxin complex (gray bars) and BoNT/B toxin complex (black bars) were added (100 μL/well of a 600 ng/ml solution) to microtiter wells coated with MAbs F1-2 and MCS6-27 and the six anti-HA70 MAbs. Data are presented as the average of two samples + one standard error.

Scotcher, M. C., Cheng, L. W., Ching, K., McGarvey, J., Hnasko, R., & Stanker, L. (2013). Development and characterization of six monoclonal antibodies to hemagglutinin-70 of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal antibodies in immunodiagnosis and immunotherapy, 32(1), 6-15.


Specifications

  • Immunogen
  • HA70-GST fusion protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • C. botulinum HA70
  • Species Reactivity
  • C. botulinum
  • Clone
  • NAP83-27-7-6
  • Applications
  • ELISA, WB

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Western Blot.

Target

  • Alternative Names
  • C. botulinum HA70; C. botulinum Non-toxin haemagglutinin HA70

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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