What specific IL-15 complex is used in this fusion?

The fusion protein complexes consist of a mutant form of IL-15 (N72D) complexed with the soluble IL-15 receptor alpha Fc domain (IL-15Ra-sFc). This complex has shown potential antitumor activity in research models.

What is the function of the BMS-936559 antibody component?

BMS-936559 is a high-affinity, fully humanized, PD-L1-specific monoclonal antibody. It inhibits the binding of PD-L1 to PD-1 and CD80, thereby blocking the immune inhibitory signal mediated by this pathway.

Anti-CD274 Antibody-Cytokine Fusion Protein, IgG (BMS-936559)-IL15/IL15Rα

CAT#: VS-0624-FY101

BMS-936559 is a high-affinity, fully humanized, PD-L1-specific monoclonal antibody that inhibits the binding of PD-L1 to PD-1 and CD80. Long-acting forms of the cytokine interleukin (IL)-15 and fusion protein complexes consisting of a mutant form of IL-15, in which asparagine at amino acid 72 (N72D) is replaced by aspartic acid (IL-15N72D), complexed with the soluble IL-15 receptor alpha (IL-15 Ralpha; IL-15Ra) Fc domain (IL-15Ra-sFc) (IL-15N72D/IL-15Ra-sFc), have potential antitumor activity.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Target
CD274

Molecule Class
IgG-IL15/IL15Rα

Species Reactivity
Human

Clone
BMS-936559

Related Disease
Cancer

Cytokine 1
IL15/IL15Rα

Cytokines 1 Description
Interleukin (IL)-15-N72D superagonist-complexed with IL-15RαSushi-Fc fusion protein has been reported to exhibit significant anti-tumor activity in murine myeloma, rat bladder cancer, and murine glioblastoma models.

Cytokine 1 Species
Human

Applications
This product is for immuno therapy research. It can be used therapeutically to deliver biologically active ligands to a desired tissue.

Target

Introduction
CD274 (CD274 molecule) is a protein-coding gene. Diseases associated with CD274 include combined lymphoma and tracheal carcinoma. Its associated pathways include ALK signaling and phosphorylation of CD3 and TCR zeta chain. An important homolog of this gene is PDCD1LG2.

Alternative Names
CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1

Gene ID
29126

UniProt ID
Q9NZQ7

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Breast cancer biomarkers at key points during disease progression

Effective Superagonist Complex

The IL-15N72D/IL-15Ra-sFc superagonist complex in this fusion protein is incredibly potent. Combined with the BMS-936559 antibody, it has shown significant antitumor activity in our murine myeloma and rat bladder cancer models.

Blocks PD-L1 Interaction

This product effectively inhibits the binding of PD-L1 to PD-1 and CD80. We confirmed this interaction blockade in vitro, and the long-acting form of the cytokine provides sustained signaling for our immune activation experiments.

Q&As

What specific IL-15 complex is used in this fusion?

A: The fusion protein complexes consist of a mutant form of IL-15 (N72D) complexed with the soluble IL-15 receptor alpha Fc domain (IL-15Ra-sFc). This complex has shown potential antitumor activity in research models.
What is the function of the BMS-936559 antibody component?

A: BMS-936559 is a high-affinity, fully humanized, PD-L1-specific monoclonal antibody. It inhibits the binding of PD-L1 to PD-1 and CD80, thereby blocking the immune inhibitory signal mediated by this pathway.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-0624-FY101.pdf

MSDS

MSDS - VS-0624-FY101.pdf

COA

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CAT
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MOB-1814z	Mouse Anti-CD274 Recombinant Antibody (clone 7A5)	FC, IHC, FuncS	Mouse IgG2b

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PSBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment	WB, ELISA, FuncS	Human scFv

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PFBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); Fab Fragment	WB, ELISA, FuncS	Human Fab

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TAB-0190CL	Anti-Human CD274 Recombinant Antibody (PD1-AB1)	FC, ELISA, FuncS

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TAB-0262CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0262CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Mouse IgG

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TAB-0263CL	Human Anti-CD274 Recombinant Antibody (TAB-0263CL)	ELISA, WB, SPR, FuncS	Humanized IgG

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TAB-0264CL	Human Anti-CD274 Recombinant Antibody (TAB-0264CL)	ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib	Humanized IgG

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TAB-0265CL	Human Anti-CD274 Recombinant Antibody (TAB-0265CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

CAT	Product Name	Application	Type
TAB-0266CL	Human Anti-CD274 Recombinant Antibody (TAB-0266CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

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TAB-0267CL	Human Anti-CD274 Recombinant Antibody (TAB-0267CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

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TAB-0992CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL)	Inhib, FC, SPR	Mouse IgG

CAT	Product Name	Application	Type
TAB-0993CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL)	FC, SPR	Mouse IgG

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TAB-0994CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL)	ELISA, FC	Mouse IgG

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TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ

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TAB-0997CL	Anti-Human CD274 Recombinant Antibody (B60-55)	ELISA, FuncS, FC, PK	Human antibody

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TAB-1002CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL)	ELISA, FC	Mouse IgG, κ

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TAB-1003CL	Human Anti-CD274 Recombinant Antibody (TAB-1003CL)	FC, ELISA	Human IgG

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TAB-1004CL	Human Anti-CD274 Recombinant Antibody (TAB-1004CL)	FC	Human IgG

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TAB-1009CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL)	WB, IHC	Mouse IgG1, κ

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TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv

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TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

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MOB-2354MZ	Mouse Anti-CD274 Recombinant Antibody (clone NJI3)	FC	Mouse IgG1

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NEUT-324CQ	Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6)	IHC-P, FC, Neut, ICC	Mouse IgG2b, κ

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NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ

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NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ

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NEUT-327CQ	Mouse Anti-CD274 Recombinant Antibody (clone MIH1)	FC, FuncS, Neut, IHC, IF	Mouse IgG1, κ

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NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2

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NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ

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NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

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NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ

CAT	Product Name	Application	Type
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

CAT	Product Name	Application	Type
MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG

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MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG

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MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG

CAT	Product Name	Application	Type
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

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HPAB-0193-YC	Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC)	ELISA	Humanized IgG4, κ

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HPAB-0194-YC	Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC)	ELISA	Mouse IgG

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HPAB-0193-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
HPAB-0194-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P))	ELISA	Mouse scFv

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HPAB-0195-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P))	SPR	Humanized scFv

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HPAB-0196-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P))	FC	Human scFv

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HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab

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HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab

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HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab

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HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab

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HPAB-N0408-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH)	ELISA, FC, BL	Human VHH

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AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

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HPAB-N0307-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH)	ELISA, FC, BL	Humanized VHH

CAT	Product Name	Application	Type
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH

CAT	Product Name	Application	Type
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH

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VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer

CAT	Product Name	Application	Type
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

CAT	Product Name	Application	Type
VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

CAT	Product Name	Application	Type
VS-0825-YC8	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
VS-0825-YC47	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47)	ELISA, IHC, FC, IP, IF, Inhib	Human IgG1 kappa

CAT	Product Name	Application	Type
VS-1025-YC9	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9)	ISZ, Cyt, FuncS

CAT	Product Name	Application	Type
VS-1025-YC39	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC39)	ISZ, Cyt, FuncS

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