What applications is this antibody recommended for?

This antibody is recommended for use in Enzyme-linked Immunosorbent Assay (ELISA) and Western Blot (WB). It is a Human IgG antibody purified by Protein A/G affinity chromatography.

Human Anti-CD274 Recombinant Antibody (clone BMS-936559)

CAT#: FAMAB-0129WJ

This product is a fully human monoclonal antibody, binds to PD-L1 on pancreatic tumor cells and prevents its association with its receptor PD-1 resulting in an anti-tumor immune environment in vitro. This antibody can be used in applications, such as ELISA, WB.

Published Data Gene Expression

SPR

Figure 1 The binding kinetics of BMS-936559.

The binding kinetics of BMS-936559 under different pH conditions was monitored by using SPR.

Liu, H., Bi, X., Zhou, Y., Shi, R., Yao, S., Qi, J., ... & Tan, S. (2020). Identification of a hotspot on PD-L1 for pH-dependent binding by monoclonal antibodies for tumor therapy. Signal transduction and targeted therapy, 5(1), 1-3.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Host Species
Human

Type
Human IgG

Antibody Isotype
IgG

Specificity
Human CD274

Species Reactivity
Human

Clone
BMS-936559

Applications
ELISA, WB

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1

Gene ID
29126

UniProt ID
Q9NZQ7

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Breast cancer biomarkers at key points during disease progression

Effective PD-L1 Detection

We used this fully human monoclonal antibody (Clone BMS-936559) for ELISA detection of CD274. It bound strongly to PD-L1. Our in vitro studies confirmed that it prevents the association with PD-1, making it a powerful tool for studying immune checkpoints.

Works Well In Western Blot

This antibody performed well in Western Blot analysis of pancreatic tumor cell lysates. It clearly identified the CD274 protein. The recombinant production ensures that we have a consistent supply of this critical reagent for our tumor microenvironment research.

Q&As

How does this antibody interact with PD-L1?

A: This fully human monoclonal antibody (clone BMS-936559) binds to PD-L1 (CD274) on tumor cells and prevents its association with its receptor PD-1. This action results in an anti-tumor immune environment in vitro.
What applications is this antibody recommended for?

A: This antibody is recommended for use in Enzyme-linked Immunosorbent Assay (ELISA) and Western Blot (WB). It is a Human IgG antibody purified by Protein A/G affinity chromatography.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - FAMAB-0129WJ.pdf

MSDS

MSDS - FAMAB-0129WJ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ

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MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG

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HPAB-0193-YC	Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC)	ELISA	Humanized IgG4, κ

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HPAB-0194-YC	Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC)	ELISA	Mouse IgG

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HPAB-0193-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P))	ELISA	Human scFv

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HPAB-0194-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P))	ELISA	Mouse scFv

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HPAB-0195-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P))	SPR	Humanized scFv

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HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab

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HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab

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HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab

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HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab

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HPAB-N0408-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH)	ELISA, FC, BL	Human VHH

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HPAB-N0307-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH)	ELISA, FC, BL	Humanized VHH

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NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH

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VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer

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VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

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VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

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VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

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VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

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VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

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VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

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VS-0825-YC8	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
VS-0825-YC47	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47)	ELISA, IHC, FC, IP, IF, Inhib	Human IgG1 kappa

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VS-1025-YC9	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9)	ISZ, Cyt, FuncS

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