Human Anti-CD274 Recombinant Antibody (clone MPDL3280A)

Name: Human Anti-CD274 Recombinant Antibody (clone MPDL3280A)
SKU: HPAB-M0361-YC
Rating: 5 (1 reviews)

CAT#: HPAB-M0361-YC

Provided is a new anti-human PDL1 antibody and can be used in medicine and biology.

Published Data Gene Expression

Figure 1 Mean (± SD) serum concentration-time profiles following a single IV administration of 0.5, 5, and 20 mg·kg−1 MPDL3280A to cynomolgus monkeys.

N = 4 animals per group. The minimum quantifiable concentration (MQC) in the MPDL3280A concentration ELISA was 0.075 μg·mL−1. Values below MQC were interpreted as missing for summary statistics.

Deng, R., Bumbaca, D., Pastuskovas, C. V., Boswell, C. A., West, D., Cowan, K. J., ... & Koeppen, H. (2016, April). Preclinical pharmacokinetics, pharmacodynamics, tissue distribution, and tumor penetration of anti-PD-L1 monoclonal antibody, an immune checkpoint inhibitor. In MAbs (Vol. 8, No. 3, pp. 593-603). Taylor & Francis.

IHC

Figure 2 PD-L1 prevalence and response rates in patients with UBC.

Representative images (320 magnification) of PD-L1 IHC staining of tumours from patients with UBC. c, Response rates, including overall response, stable disease and progressive disease by tumour-infiltrating immune cell PD-L1 IHC status. Includes both confirmed
and unconfirmed responses per RECIST v1.1. Five of seventeen responses were unconfirmed. Best response is not known for seven patients.

Powles, T., Eder, J. P., Fine, G. D., Braiteh, F. S., Loriot, Y., Cruz, C., ... & Shen, X. (2014). MPDL3280A (anti-PD-L1) treatment leads to clinical activity in metastatic bladder cancer. Nature, 515(7528), 558-562.

FuncS

Figure 3 Antitumour activity of MPDL3280A by immunohistochemistry (IHC) tumour-infiltrating immune cell (IC) and biomarker status.

Kaplan-Meier curve showing the phase I percentage of progression-free survival by patient IHC (IC) status. Censored data are indicated by vertical tick marks. CI, confidence interval; NSCLC, non-small cell lung cancer; ORR, objective response rate; PD, progressive disease; PFS, progression free survival; PR, partial response; RECIST, Response Evaluation Criteria in Solid Tumours; SD, stable disease.

Herbst, R. S., Soria, J. C., Kowanetz, M., Fine, G. D., Hamid, O., Gordon, M. S., ... & Kohrt, H. E. (2014). Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature, 515(7528), 563-567.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Host Species
Human

Type
Human IgG4, κ

Specificity
Human CD274

Species Reactivity
Human

Clone
MPDL3280A

Applications
PK, IHC, FuncS, ELISA

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The CD274 antibody has been reported in applications of Pharmacokinetic, Immunohistochemistry, Functional Assay, Enzyme-linked Immunosorbent Assay.

Target

Alternative Names
CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1;

Gene ID
29126

UniProt ID
Q9NZQ7

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-M0361-YC.pdf

MSDS

MSDS - HPAB-M0361-YC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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HPAB-0197-YC	Rabbit Anti-CD274 Recombinant Antibody (HPAB-0197-YC)	ELISA, FC	Rabbit IgG
HPAB-0198-YC	Mouse Anti-CD274 Recombinant Antibody (HPAB-0198-YC)	ELISA, FC, Block	Mouse IgG
HPAB-0199-YC	Human Anti-CD274 Recombinant Antibody (HPAB-0199-YC)	ELISA, FC, Block	Chimeric (mouse/human) IgG4, κ

CAT	Product Name	Application	Type
TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ
TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv
TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
TAB-0262CL-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0262CL-S(P))	ELISA, WB, SPR, FuncS	Mouse scFv
TAB-0262CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0262CL-F(E))	ELISA, WB, SPR, FuncS	Mouse Fab
TAB-1009CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1009CL-F(E))	WB, IHC	Mouse Fab
TAB-1010CL-F(E)	Anti-Human CD274 Recombinant Antibody Fab Fragment	FC
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CAT	Product Name	Application	Type
TAB-0263CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0263CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0264CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0264CL-F(E))	ELISA, WB, SPR, Inhib, FuncS	Humanized Fab
TAB-0265CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0265CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0266CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0266CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0267CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0267CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab

CAT	Product Name	Application	Type
TAB-1003CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1003CL-F(E))	FC, ELISA	Human Fab
TAB-1004CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1004CL-F(E))	FC	Human Fab
TAB-1005CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1005CL-F(E))	FC	Human Fab
TAB-1006CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1006CL-F(E))	FC	Human Fab
TAB-1007CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1007CL-F(E))	FC	Human Fab

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NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ
NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ
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CAT	Product Name	Application	Type
NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2
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NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

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MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG
MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG
MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0197-YC-S(P)	Rabbit Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0197-YC-S(P))	ELISA, FC	Rabbit scFv
HPAB-0198-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0198-YC-S(P))	ELISA, FC, Block	Mouse scFv
HPAB-0199-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0199-YC-S(P))	ELISA, FC, Block	Mouse scFv
HPAB-0200-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0200-YC-S(P))	ELISA, FC, Block	Humanized scFv
HPAB-0201-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0201-YC-S(P))	ELISA, FC, Block, FuncS	Human scFv

CAT	Product Name	Application	Type
HPAB-0400LY-F(E)	Human Anti-PDCD1 Recombinant Antibody; Fab Fragment (HPAB-0400LY-F(E))	ELISA, RIA, WB	Human Fab
HPAB-0401LY-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0401LY-F(E))	ELISA, RIA, WB	Human Fab
HPAB-0402LY-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0402LY-F(E))	ELISA, RIA, WB	Human Fab
HPAB-0403LY-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0403LY-F(E))	ELISA, RIA, WB	Human Fab
HPAB-0404LY-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0404LY-F(E))	ELISA, RIA, WB	Human Fab

CAT	Product Name	Application	Type
AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

CAT	Product Name	Application	Type
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH
NBD-0067-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH)	ELISA, FC	Camelid VHH
NBD-0068-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0068-YJ-VHH)	ELISA, FC	Camelid VHH
NBD-0069-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0069-YJ-VHH)	ELISA, FC	Camelid VHH

CAT	Product Name	Application	Type
VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

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VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC
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VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

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