Does this antibody detect recombinant CD274?

Yes, this recombinant human antibody (clone Q2-20) recognizes both natural and recombinant forms of human CD274.

What is the source of this antibody?

This antibody was derived from a phage display library. It is a Human IgG1 antibody purified to over 95% purity.

Human Anti-CD274 Recombinant Antibody (clone Q2-20)

CAT#: HPAB-0506YY

This product is a recombinant human antibody that recognizes (both natural and recombinant) human CD274.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Host Species
Human

Derivation
Phage display library

Type
Human IgG1

Specificity
Human

Species Reactivity
Human

Clone
Q2-20

Applications
ELISA

Related Disease
Cancer

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in ELISA.

Target

Alternative Names
CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1;

Gene ID
29126

UniProt ID
Q9NZQ7

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Breast cancer biomarkers at key points during disease progression

Reliable CD274 Antibody

We used clone Q2-20 for our research on immune inhibitory receptors. It is a recombinant human antibody that recognizes human CD274 perfectly. The fact that it detects both natural and recombinant forms of the protein made it very versatile for our ELISA experiments.

Excellent Purity And Binding

The purity of this antibody was stated as greater than 95% by HPLC, and our results reflect that quality. We saw very little background in our assays. It is derived from a phage display library, which is an interesting technology that seems to produce high-affinity binders.

Q&As

Does this antibody detect recombinant CD274?

A: Yes, this recombinant human antibody (clone Q2-20) recognizes both natural and recombinant forms of human CD274.
What is the source of this antibody?

A: This antibody was derived from a phage display library. It is a Human IgG1 antibody purified to over 95% purity.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0506YY.pdf

MSDS

MSDS - HPAB-0506YY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone Q2-20"

CAT
Product Name

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MOB-1814z	Mouse Anti-CD274 Recombinant Antibody (clone 7A5)	FC, IHC, FuncS	Mouse IgG2b

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PABL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006)	WB, ELISA, FuncS	Human IgG

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PSBL-298	Human Anti-CD274 Recombinant Antibody (clone CBLC-006); scFv Fragment	WB, ELISA, FuncS	Human scFv

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TAB-0263CL	Human Anti-CD274 Recombinant Antibody (TAB-0263CL)	ELISA, WB, SPR, FuncS	Humanized IgG

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TAB-0264CL	Human Anti-CD274 Recombinant Antibody (TAB-0264CL)	ELISA, WB, SPR, ADCC, CDC, FuncS, Inhib	Humanized IgG

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TAB-0265CL	Human Anti-CD274 Recombinant Antibody (TAB-0265CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

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TAB-0266CL	Human Anti-CD274 Recombinant Antibody (TAB-0266CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

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TAB-0267CL	Human Anti-CD274 Recombinant Antibody (TAB-0267CL)	ELISA, WB, SPR, ADCC, CDC, FuncS	Humanized IgG

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TAB-0992CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL)	Inhib, FC, SPR	Mouse IgG

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TAB-0993CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0993CL)	FC, SPR	Mouse IgG

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TAB-0994CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0994CL)	ELISA, FC	Mouse IgG

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TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ

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TAB-0997CL	Anti-Human CD274 Recombinant Antibody (B60-55)	ELISA, FuncS, FC, PK	Human antibody

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TAB-1002CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1002CL)	ELISA, FC	Mouse IgG, κ

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TAB-1003CL	Human Anti-CD274 Recombinant Antibody (TAB-1003CL)	FC, ELISA	Human IgG

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TAB-1004CL	Human Anti-CD274 Recombinant Antibody (TAB-1004CL)	FC	Human IgG

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TAB-1009CL	Mouse Anti-CD274 Recombinant Antibody (TAB-1009CL)	WB, IHC	Mouse IgG1, κ

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TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv

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TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

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MOB-2354MZ	Mouse Anti-CD274 Recombinant Antibody (clone NJI3)	FC	Mouse IgG1

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NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ

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NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ

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NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2

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NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ

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NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

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NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ

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NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

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MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG

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MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG

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MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG

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MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

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HPAB-0193-YC	Human Anti-CD274 Recombinant Antibody (HPAB-0193-YC)	ELISA	Humanized IgG4, κ

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HPAB-0194-YC	Mouse Anti-CD274 Recombinant Antibody (HPAB-0194-YC)	ELISA	Mouse IgG

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HPAB-0193-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P))	ELISA	Human scFv

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HPAB-0194-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P))	ELISA	Mouse scFv

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HPAB-0195-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P))	SPR	Humanized scFv

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HPAB-0196-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P))	FC	Human scFv

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HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab

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HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab

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HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab

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HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab

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HPAB-N0408-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH)	ELISA, FC, BL	Human VHH

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AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody

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AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

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HPAB-N0307-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0307-YC-VHH)	ELISA, FC, BL	Humanized VHH

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NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH

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NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH

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VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer

CAT	Product Name	Application	Type
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

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VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

CAT	Product Name	Application	Type
VS-0825-YC8	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC8)	ELISA	Mouse IgG

CAT	Product Name	Application	Type
VS-0825-YC47	SmartAb™ Recombinant Anti-CD274 pH-dependent Antibody (VS-0825-YC47)	ELISA, IHC, FC, IP, IF, Inhib	Human IgG1 kappa

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VS-1025-YC9	Anti-CD274 Antibody Prodrug, Protease Activated (VS-1025-YC9)	ISZ, Cyt, FuncS

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