Mouse Anti-CD33 Recombinant Antibody (clone HIM3-4) (CAT#: VS3-CJ761)

• Immunogen
• Native purified human CD33

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human CD33

• Species Reactivity
• Human

• Clone
• HIM3-4

• Applications
• IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for one year. Do not freeze.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunofluorescence, Flow Cytometry.

• Alternative Names
• p67; SIGLEC3; SIGLEC-3

• Gene ID
• 945

• UniProt ID
• P20138

• Sequence Similarities
• Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

• Cellular Localization
• Cell membrane, Membrane, Peroxisome

• Post Translation Modifications
• Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition.
  Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.

• Protein Refseq
• NP_001076087.1; NP_001171079.1; NP_001763.3

• Function
• Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212).
  Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872).
  Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109).
  These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109).
  In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109).
  One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS