Rabbit Anti-CD80 Recombinant Antibody (clone 3C3) (CAT#: ZG-0595J)
This product is a rabbit antibody that recognizes CD80.
Specific Inquiry
WB
Figure 1 Rabbit Anti-CD80 Antibody (ZG-0595J) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD80 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 30, 19 kDa
Observed band size: 60 kDa
IHC
Figure 2 Rabbit Anti-CD80 Antibody (ZG-0595J) in IHC
IHC image of ZG-0595J diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 3 Rabbit Anti-CD80 Antibody (ZG-0595J) in IP
Immunoprecipitating CD80 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0595J in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0595J(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human CD80
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD80
- Species Reactivity
- Human
- Clone
- 3C3
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1; h1F1; hu1F1; humanized 1F1
- Gene ID
- 941
- UniProt ID
- P33681
Related Resources
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Autoimmune Thyroid Disease
Graft-versus-Host Disease
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-215 | Anti-Human CD80 Recombinant Antibody (Galiximab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - lambda |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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