Camelid Anti-CHIKV E1 Recombinant Antibody (clone CC3) (CAT#: FAMAB-0131WJ)

Figure 1 MagPlex sandwich assay for CHIKV VLPs using sdAb as captures and tracers

Five sdAb were immobilized onto the surface of MagPlex microspheres and serve to capture various VLP concentrations. Bt-CC3 was used as a tracer sdAb in A while Bt-CA6 was used in B.

Figure 2 ELISA assay for CHIKV VLPs using sdAb and anti-E2 mAb, CHK48, as capture reagents and tracers.

CHK48, Bt-CA6 and Bt-CC3 were used as tracers in all the reactions.

Figure 3 Dose dependent neutralization of CHIKV using plaque reduction neutralization test (PRNT).

Vero cells were used to measure the plaque numbers after the incubation of CHIKV and two sdAb, CC3 and CA6.

Figure 4 CC3-AP (B) and CA6-AP (C) were used as primary antibodies to blot against CHIKV VLP.

The arrows indicate the proteins rE1, rE2 as well as E1 and E2 in the VLP.