Mouse Anti-CNOT4 Recombinant Antibody (VS3-CJ54) (CAT#: VS3-CJ54)

• Immunogen
• Recombinant full length of human CNOT4

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human CNOT4

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• CLONE243; NOT4; NOT4H

• Gene ID
• 4850

• UniProt ID
• O95628

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Autoubiquitinated.

• Protein Refseq
• NP_001008226.1; NP_001177776.1; NP_001177777.1

• Function
• Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292).
  Involved in activation of the JAK/STAT pathway (PubMed:11823428, PubMed:22159038).
  Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292).
  Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed:29861391).
  As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy (PubMed:29861391).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS