Mouse Anti-Crotoxin Recombinant Antibody (clone A10G) (CAT#: FAMAB-0331JF)

Recombinant monoclonal antibody specially binds to crotoxin. A10G is a mouse monoclonal antibody that can be useful in the immune passive therapy of snakebite victims.


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  • Published Data
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Quantification of anti-crotoxin mAb by ELISA.

Figure 1 Quantification of anti-crotoxin mAb by ELISA.

Different concentrations of mAbs were assayed by ELISA for its crotoxin reactivity.

Cardoso, D. F., Nato, F., England, P., Ferreira, M. L., Vaughan, T. J., Mota, I., ... & Lafaye, P. (2000). Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library. Scandinavian journal of immunology, 51(4), 337-344.

Inhib

Figure 2 Inhibition assays were performed to measure the ability of different scFvs to compete with mouse anti-CB MoAbs for binding to crotoxin.

Figure 2 Inhibition assays were performed to measure the ability of different scFvs to compete with mouse anti-CB MoAbs for binding to crotoxin.

Each MoAb recognizes a specific epitopic region on the CB subunit.

Cardoso, D. F., Nato, F., England, P., Ferreira, M. L., Vaughan, T. J., Mota, I., ... & Lafaye, P. (2000). Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library. Scandinavian journal of immunology, 51(4), 337-344.

WB

Figure 3 Cross-reactivity of anti-crotoxin mAb.

Figure 3 Cross-reactivity of anti-crotoxin mAb.

Specificity analysis of the VHHs with CB by Western blot.

Luiz, M. B., Pereira, S. S., Prado, N. D., Gonçalves, N. R., Kayano, A. M., Moreira-Dill, L. S., ... & Zuliani, J. P. (2018). Camelid single-domain antibodies (VHHs) against crotoxin: A basis for developing modular building blocks for the enhancement of treatment or diagnosis of crotalic envenoming. Toxins, 10(4), 142.


Specifications

  • Immunogen
  • Equal volumes of crotoxin basic subunit in H2O and titerMax adjuvant
  • Host Species
  • Mouse
  • Derivation
  • Mouse phage display scFv library
  • Type
  • Mouse IgG
  • Specificity
  • Crotalus durissus terrificus crotoxin
  • Species Reactivity
  • Crotalus durissus terrificus
  • Clone
  • A10G
  • Applications
  • WB, ELISA, Inhib
  • Related Disease
  • Snakebite victims

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Crotoxin antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Inhibition.

Target

  • Alternative Names
  • Crotoxin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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