Recombinant Mouse Anti-DENV EDIII Antibody (1G6) (CAT#: HPAB-0003-WJ)
This product is a mouse monoclonal antibody that recognizes dengue virus target. The antibody clone reacts with the epitope (aa 387-390) of DENV4 ED3. Neutralizing activity in vitro Experimental results show that anti-dengue virus antibody 1G6 4 can effectively block the 4 dengue virus infection of BHK-21 cells and this antibody may be used for the preparation of a medicament for treating dengue virus type-4 infection.
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WB
Figure 1 Characterization of mAb 1G6 in vitro.
(B) Binding of mAb 1G6 with purified DENV1-4 ED3 proteins by western blotting. The cross-reactive mAb 6E1 was used as a positive control.
Ji, G. H., Deng, Y. Q., Yu, X. J., Jiang, T., Wang, H. J., Shi, X., ... & Dai, J. X. (2015). Characterization of a novel dengue serotype 4 virus-specific neutralizing epitope on the envelope protein domain III. PloS one, 10(10), e0139741.
IF
Figure 2 Characterization of mAb 1G6 in vitro.
(A) Binding of mAb 1G6 with DENV1-4 by IFA. DENV antigen was detected on day 3 after infection with DENV1-4 with mAb 1G6 and an FITC-conjugated secondary antibody. Cells were counterstained with Evan's blue, resulting in red fluorescence of cells.
Ji, G. H., Deng, Y. Q., Yu, X. J., Jiang, T., Wang, H. J., Shi, X., ... & Dai, J. X. (2015). Characterization of a novel dengue serotype 4 virus-specific neutralizing epitope on the envelope protein domain III. PloS one, 10(10), e0139741.
Neut
Figure 3 MAb 1G6 mediated neutralization in vitro through blocking viral adsorption.
MAbs with concentrations of 500, 100, 20, 4, 0.8μg/ml were mixed with constant 50 PFU viruses. The results were expressed as percentage of neutralization (% Neutralization) shown with standard deviations. (A) Neutralizing ability of mAb 1G6 against DENV4. The non-neutralizing mAb 6E1 was used as a negative control.
Ji, G. H., Deng, Y. Q., Yu, X. J., Jiang, T., Wang, H. J., Shi, X., ... & Dai, J. X. (2015). Characterization of a novel dengue serotype 4 virus-specific neutralizing epitope on the envelope protein domain III. PloS one, 10(10), e0139741.
ELISA
Figure 4 Epitope mapping of 1G6.
(A) Unique mAb 1G6-specific phages after three rounds of bio-panning. The binding of isolated phage clones to 1G6 was examined by ELISA. Data represent the mean ± S.D. of duplicate measurements.
Ji, G. H., Deng, Y. Q., Yu, X. J., Jiang, T., Wang, H. J., Shi, X., ... & Dai, J. X. (2015). Characterization of a novel dengue serotype 4 virus-specific neutralizing epitope on the envelope protein domain III. PloS one, 10(10), e0139741.
Specifications
- Immunogen
- Prokaryotic expressed ED3 tandem protein
- Host Species
- Mouse
- Derivation
- Hybridomas
- Type
- Mouse IgG
- Specificity
- Dengue virus serotype 4 ED3
- Species Reactivity
- Dengue virus
- Clone
- 1G6
- Applications
- IF, WB, ELISA, Neut
- Related Disease
- Dengue virus type-4 infection
Applications
- Application Notes
- The DENV EDIII antibody has been reported in applications of Immunofluorescence, Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization.
Target
- Alternative Names
- Dengue virus; DENV; dengue virus E-DIII; DENV E-DIII
- Function
- Blocking
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0003-WJ-S(P) | Recombinant Mouse Anti-DENV EDIII Antibody scFv Fragment (1G6) | IF, WB, ELISA, Neut | Mouse scFv |
| FAMAB-0151WJ-S(P) | Mouse Anti-DENV EDIII Recombinant Antibody (clone EDIIImAb-61); scFv Fragment | IF, ELISA, Neut | Mouse scFv |
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