CytoStream™ Rabbit Anti-EN1 Recombinant Antibody (VS13-YC357) (CAT#: VS13-YC357)
The CytoStream™ Rabbit Anti-EN1 Recombinant Antibody is specific for human, mouse and rat EN1. EN1, a homeobox gene, regulates developmental patterning in systems like the CNS, with mutations linked to segmental defects affecting organismal structuring. The anti-EN1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14884/620_F1_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14884/620_F1_4_selected.jpg
Figure 2 Low expression in placenta
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73141/159692_A_2_7_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73141/159692_A_2_7_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73141/159692_B_8_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73141/159692_B_8_5.jpg
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC, PC-3, U-138 MG, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163064-EN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163064-EN1
Specifications
- Immunogen
- Recombinant human Engrailed 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EN1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IP, FC
- Conjugate
- NONE
- Related Disease
- Endove Syndrome, Limb-Brain Type and Eccrine Porocarcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 10 mM PBS (pH 7.4) containing 150 mM NaCl, 50% Glycerol and 0.05% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Engrailed Homeobox 1; Homeobox Protein En-1; Hu-En-1; Homeobox Protein Engrailed-1; Engrailed Homolog 1;
- Gene ID
- 2019
- UniProt ID
- Q05925
- Long Name
- Engrailed Homeobox 1
- Sequence Similarities
- Belongs to the engrailed homeobox family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001417.3
- Function
- Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EN1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0744MZ | Recombinant Mouse Anti-Human EN1 Antibody | ELISA | Mouse antibody |
| VS3-FY1708 | Rabbit Anti-EN1 Recombinant Antibody (clone R02-8D2) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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