Mouse Anti-ETF1 Recombinant Antibody (clone 4F9H12) (CAT#: VS7-0425-WR778)

• Immunogen
• Recombinant human RF1 (AA: 288-437) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human ETF1

• Species Reactivity
• Human

• Clone
• 4F9H12

• Molecule Class
• Class 1 Release Factor

• Applications
• WB, FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1

• Gene ID
• 2107

• UniProt ID
• ERF1

• Long Name
• Eukaryotic Translation Termination Factor 1

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Acetylation; Hydroxylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation

• Protein Refseq
• NP_001243231.1

• Function
• Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed:10676813, PubMed:16777602, PubMed:24486019, PubMed:26245381, PubMed:27863242, PubMed:36638793, PubMed:7990965).; The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:26245381, PubMed:27863242, PubMed:36638793).; ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed:26245381, PubMed:27863242, PubMed:36638793).; Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed:10676813, PubMed:16777602, PubMed:26245381, PubMed:27863242).; Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:19417104).; Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS