Mouse Anti-HIV-1 Rev Recombinant Antibody (clone 2G2) (CAT#: MRO-4302CQ)

Recombinant mouse antibody to Rev. 2G2 does not recognize any of the fusion proteins, and may be directed against a conformational epitope.


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Inhib

Figure 1 Inhibition of nuclear export of GFP–Rev–NES–GST fusion protein by antiactin antibodies in cultured Vero cells.

Figure 1 Inhibition of nuclear export of GFP–Rev–NES–GST fusion protein by antiactin antibodies in cultured Vero cells.

Micro-injected cells are denoted by arrows. Bar, 20um.

Hofmann, W., Reichart, B., Ewald, A., Müller, E., Schmitt, I., Stauber, R. H., ... & Dabauvalle, M. C. (2001). Cofactor requirements for nuclear export of rev response element (RRE)–and constitutive transport element (CTE)–containing retroviral RNAs: an unexpected role for actin. The Journal of cell biology, 152(5), 895-910.

WB

Figure 2 Antibodies against actin inhibit nuclear export of GST–Rev–NES, GST–PKI–NES, RRE RNA, and CTE RNA in
 Xeno- pus
 oocytes.

Figure 2 Antibodies against actin inhibit nuclear export of GST–Rev–NES, GST–PKI–NES, RRE RNA, and CTE RNA in Xeno- pus oocytes.

The nuclear localization of the nonexportable U6 RNA confirms the accuracy of the nuclear injections.

Hofmann, W., Reichart, B., Ewald, A., Müller, E., Schmitt, I., Stauber, R. H., ... & Dabauvalle, M. C. (2001). Cofactor requirements for nuclear export of rev response element (RRE)–and constitutive transport element (CTE)–containing retroviral RNAs: an unexpected role for actin. The Journal of cell biology, 152(5), 895-910.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Recognizes HIV-1 Rev
  • Species Reactivity
  • HIV-1
  • Clone
  • 2G2
  • Applications
  • Inhib, WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 Rev antibody has been reported in applications of Inhibition, Western Blot.

Target

  • Alternative Names
  • Rev; p19; HIV-1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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