The antibody is a a chimeric mouse/human MAb against the human IL-2R (Interleukin-2 receptor). It binds to a different epitope on p.55 than IL-2 and the chimeric antibodies interacted more efficiently than the murine antibody present in the PBMC cultures. This product has been determined using Enzyme-linked immunosorbent assay, Flow cytometry and Immunoprecipitation.
Figure 1 Binding compasons of 125I radiolabeled AHT107 antibodies.
PMA stimulated CCRF-GEM cells were incubated with antibodies for 30min at 37°C.
Figure 2 Chimeric AHT107 live cell IL-2R binding assay.
Cell bound antibodies were detected with HRP-labeled goat anti-human IgG. FACScan analysis with PE-anti-CD25 confirmed preferential expression of IL-2R on stimulated CCRF-CEM cells.
Figure 3 Effect of AHT107 antibodies on appearance of IL-2R positive cells.
PBMC were stimulated in a MLR for 4 d in the presence of 10 ug/ml antibodies. Cells were stained with PE-anti-CD25 IL-2R positive cells were identified in a flow cytometer and correspond to the upper left quadrant of the histogram.
Figure 4 Effect of AHT107 antibodies on proliferation of PBMC cells.
Stimulated cells were analyzed for proliferation by incorporation of 3H-thymidine into DNA.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0155MZ | Recombinant Mouse Anti-IL-2R Antibody (clone OR-73) | FC | Mouse antibody |
FAMAB-0180YC | Mouse Anti-IL2R Recombinant Antibody (clone AHT107) | ELISA, FC, IP | Mouse IgG1 |
FAMAB-0196CQ | Mouse Anti-IL2R Recombinant Antibody (clone mAb1) | ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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