Recombinant Llama Anti-MARV NP Single Domain Antibody (PABC-561)
CAT#: PABC-561
This product is a recombinant Llama antibody that can recognize MARV NP.
Tested Data
Published Data
SDS-PAGE
Figure 1 Recombinant Llama Anti-MARV NP Single Domain Antibody (B) (PABC-561) in SDS-PAGE
SDS-PAGE analysis of PABC-561 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABC-561 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Recombinant Llama Anti-MARV NP Single Domain Antibody (B) (PABC-561) in HPLC
The purity of PABC-561 was greater than 95% as determined by SEC-HPLC.
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Figure 1 Titrations of fusions of nluc–NP C-terminal domains of either MARV or MLAV over oriented sdAb A, B or C.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
ELISA
Figure 2 ELISA titration of the anti-MARV sdAb monomers and dimers over MARV NP with the highest concentration also applied to Bundibugyo NP.
Bundibugyo NP was expressed at higher levels than Zaire NP and so was convenient to use for controls yet shares high homology at the C-terminal domain for our studies.
Darling, T. L., Sherwood, L. J., & Hayhurst, A. (2017). Intracellular crosslinking of filoviral nucleoproteins with xintrabodies restricts viral packaging. Frontiers in immunology, 8, 1197.
Figure 3 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
Purified recombinant MARV or Bundibugyo (BEBOV) NP polymers were titrated over passively immobilized anti-MARV NP-sdAb, and captured NP subsequently detected with a constant amount of each phage displayed sdAb followed by anti-M13-horseradish peroxidase (HRP) conjugate.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
WB
Figure 4 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
1,000 ng (1), 100 ng (2), or 10 ng (3) of purified MARV NP C-terminus was probed with 100 nM of each sdAb-AP fusion protein for equivalent times and developed side-by-side.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
Figure 5 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
The sdAb-gluc fusions were titrated over passively immobilized mbp-NP600 fusion protein to determine the monovalent EC50 values.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
Figure 6 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
The nluc-NP600 fusions were titrated over oriented immobilized sdAb to determine the EC50 values in a reversed orientation.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
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Specifications
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- MARV NP
- Species Reactivity
- Lake Victoria marburgvirus (strain Musoke-80)
- Clone
- PABC-561
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA. For details, refer to Published Data.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "MARV NP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0810CQ | Recombinant Mouse Anti-MARV NP Antibody (2A7) | WB, ELISA, IHC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-560 | Recombinant Llama Anti-MARV NP Single Domain Antibody (PABC-560) | ELISA, WB | Llama VHH |
| PABC-562 | Recombinant Llama Anti-MARV NP Single Domain Antibody (PABC-562) | ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1794CQ | Anti-MARV NP Recombinant Antibody (clone sdAb 4) | ELISA | Camelid VHH |
| PABS-0292 | Camelid Anti-MARV NP Recombinant Antibody (clone sdAb D) | ELISA | Camelid VHH |
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