Mouse Anti-MDI Recombinant Antibody (clone AD3) (CAT#: FAMAB-0217CQ)
This product is a recombinant mouse antibody clone AD3, which specifically binds to MDI (Methylene diphenyl diisocyanate).
Specific Inquiry
ELISA
Figure 1 New murine monoclonal antibodies specific binding to MDI-conjugated proteins.
ELISA data demonstrate the binding (O.D. value on Y-axis) of different mAbs (X-axis) to unconjugated control mock exposed proteins, MDI, HDI, TDI or FITC conjugated proteins, as labeled. Alb= albumin, Tf= transferrin, Ova=ovalbumin, MSA=mouse albumin.
Wisnewski, A. V., & Liu, J. (2013). Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular immunology, 54(2), 233-237.
WB
Figure 2 Anti-MDI mAbs recognize MDI-albumin by Western blot.
Panel (A) Different mAbs were Western blotted from a native gel (protein stain lanes 1 and 2) against MDI-albumin (odd numbered lanes) or unconjugated albumin (even numbered lanes). Lanes 1, 2 (CE2), lanes 3, 4 (AD3), lanes 5, 6 (DD10), lanes 7, 8 (AH6). *Note all 6 mAbs displayed similar (triple banding) binding patterns. Arrows highlight MDI-albumin monomer, and cross-linked dimers and trimers. Panel (B) Left side shows protein stain of SDS-PAGE, and right side shows Western blot under reducing conditions with mAb BC8. Lanes 1, 5- unconjugated albumin' lanes 2, 6- MDI-albumin prepared with 0.001% (w/v) MDI; lanes 3, 7- MDI-albumin prepared with 0.01% (w/v) MDI; lanes 4, 8- MDI-albumin prepared with 0.1% (w/v) MDI. *Note all 6 mAbs displayed similar (MDI dose-dependent) binding patterns.
Wisnewski, A. V., & Liu, J. (2013). Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular immunology, 54(2), 233-237.
ELISA
Figure 3 Cross-competition of different mAbs binding to MDI.
Competitive inhibition of biotinylated mAb AD3 (panel A) or mAb AH6 (panel B) binding to MDI-albumin by increasing concentrations of unlabeled mAbs as labeled in legend. *Note BC8 and CE2 data are overlapping in panel A.
Wisnewski, A. V., & Liu, J. (2013). Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular immunology, 54(2), 233-237.
Specifications
- Immunogen
- MDI-conjugated to autologous serum proteins
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- AD3
- Applications
- ELISA, WB
- Related Disease
- Asthma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Western Blot.
Target
- Alternative Names
- MDI; Methylene diphenyl diisocyanate
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0218CQ | Mouse Anti-MDI Recombinant Antibody (clone AH6) | ELISA, WB | Mouse IgG1, κ |
| FAMAB-0219CQ | Mouse Anti-MDI Recombinant Antibody (clone BC8) | ELISA, WB | Mouse IgG1, κ |
| FAMAB-0220CQ | Mouse Anti-MDI Recombinant Antibody (clone CE2) | ELISA, WB | Mouse IgG1, κ |
| FAMAB-0221CQ | Mouse Anti-MDI Recombinant Antibody (clone DA5) | ELISA, WB | Mouse IgG1, κ |
| FAMAB-0222CQ | Mouse Anti-MDI Recombinant Antibody (clone DD10) | ELISA, WB | Mouse IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0217CQ-F(E) | Mouse Anti-MDI Recombinant Antibody (clone AD3); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0218CQ-F(E) | Mouse Anti-MDI Recombinant Antibody (clone AH6); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0219CQ-F(E) | Mouse Anti-MDI Recombinant Antibody (clone BC8); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0220CQ-F(E) | Mouse Anti-MDI Recombinant Antibody (clone CE2); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0221CQ-F(E) | Mouse Anti-MDI Recombinant Antibody (clone DA5); Fab Fragment | ELISA, WB | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0217CQ-S(P) | Mouse Anti-MDI Recombinant Antibody (clone AD3); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0218CQ-S(P) | Mouse Anti-MDI Recombinant Antibody (clone AH6); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0219CQ-S(P) | Mouse Anti-MDI Recombinant Antibody (clone BC8); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0220CQ-S(P) | Mouse Anti-MDI Recombinant Antibody (clone CE2); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0221CQ-S(P) | Mouse Anti-MDI Recombinant Antibody (clone DA5); scFv Fragment | ELISA, WB | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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