Rabbit Anti-NCOA1 Recombinant Antibody (VS13-YC772) (CAT#: VS13-YC772)
The Rabbit Anti-NCOA1 Recombinant Antibody is specific for human NCOA1. NCOA1 encodes a transcriptional coactivator for hormone receptors, part of the p160 family, featuring spliced variants and nuclear localization. The anti-NCOA1 antibody can be used for Western blot (1/500-1/2000), Immunohistochemistry-paraffin (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70213/1586_H7_6_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70213/1586_H7_6_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70520/1398_B10_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70520/1398_B10_4_selected.jpg
Figure 3 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_B_9_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (RH-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084676-NCOA1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084676-NCOA1
Specifications
- Immunogen
- Recombinant human SRC1, aa 380-770.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NCOA1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/100
Target
- Alternative Names
- Nuclear Receptor Coactivator 1; Class E Basic Helix-Loop-Helix Protein 74; Renal Carcinoma Antigen NY-REN-52; EC 2.3.1.48; BHLHe74; RIP160; SRC1; Steroid Receptor Coactivator-1; Steroid Receptor Coactivator 1;
- Gene ID
- 8648
- UniProt ID
- Q15788
- Long Name
- Nuclear Receptor Coactivator 1
- Cellular Localization
- Nucleus
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1950z | Mouse Anti-NCOA1 Recombinant Antibody (clone 32C3) | IHC, IP, WB | Mouse IgG1 |
| MOB-0085CT | Recombinant Mouse anti-Human NCOA1 Monoclonal antibody (4643D7b) | Dot, WB | |
| MRO-1432-CN | Recombinant Rabbit Anti-NCOA1 Monoclonal Antibody (JB66-31) | WB, IHC, IP | Rabbit IgG |
| VS3-CJ31 | Mouse Anti-NCOA1 Recombinant Antibody (VS3-CJ31) | WB, ELISA | Mouse IgG |
| VS3-FY1011 | Recombinant Rabbit Anti-NCOA1 Antibody (clone R07-5B7) | WB, IHC-P, IP | Rabbit IgG |
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