Rabbit Anti-NCOA1 Recombinant Antibody (VS13-YC772) (CAT#: VS13-YC772)
The Rabbit Anti-NCOA1 Recombinant Antibody is specific for human NCOA1. NCOA1 encodes a transcriptional coactivator for hormone receptors, part of the p160 family, featuring spliced variants and nuclear localization. The anti-NCOA1 antibody can be used for Western blot (1/500-1/2000), Immunohistochemistry-paraffin (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70213/1586_H7_6_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70213/1586_H7_6_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70520/1398_B10_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70520/1398_B10_4_selected.jpg
Figure 3 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_B_9_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_8_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19402/42041_A_9_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (RH-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084676-NCOA1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084676-NCOA1
Specifications
- Immunogen
- Recombinant human SRC1, aa 380-770.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NCOA1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/100
Target
- Alternative Names
- Nuclear Receptor Coactivator 1; Class E Basic Helix-Loop-Helix Protein 74; Renal Carcinoma Antigen NY-REN-52; EC 2.3.1.48; BHLHe74; RIP160; SRC1; Steroid Receptor Coactivator-1; Steroid Receptor Coactivator 1;
- Gene ID
- 8648
- UniProt ID
- Q15788
- Long Name
- Nuclear Receptor Coactivator 1
- Cellular Localization
- Nucleus
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0085CT | Recombinant Mouse anti-Human NCOA1 Monoclonal antibody (4643D7b) | Dot, WB | |
| MRO-1432-CN | Recombinant Rabbit Anti-NCOA1 Monoclonal Antibody (JB66-31) | WB, IHC, IP | Rabbit IgG |
| VS3-WK322 | Rabbit Anti-NCOA1 Recombinant Antibody (clone JB66-31) | WB, ELISA | Rabbit IgG |
| VS3-CJ31 | Mouse Anti-NCOA1 Recombinant Antibody (VS3-CJ31) | WB, ELISA | Mouse IgG |
| VS3-FY1011 | Recombinant Rabbit Anti-NCOA1 Antibody (clone R07-5B7) | WB, IHC-P, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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