Mouse Anti-NHP PGAM2 Recombinant Antibody (clone OTI2F5)
CAT#: VS-1024-XY382
This finely tuned monoclonal antibody targets PGAM2, demonstrating exceptional reactivity across multiple species, including dog, human, mouse, non-human primate, and rat. Utilizing the full-length human recombinant protein of PGAM2 as the immunogen, it is specifically suited for applications such as Western blotting and immunofluorescence. This antibody stands as a vital resource for researchers delving into the regulatory mechanisms of PGAM2, facilitating deeper explorations into its biological functions and implications in metabolic pathways and disease states.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43801/1834_G1_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50314/1561_C10_3_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60173/139682_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60173/139682_A_4_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60173/139682_B_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (SCLC-21H)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164708-PGAM2
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Specifications
- Immunogen
- Full length human recombinant protein of PGAM2
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- PGAM2
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI2F5
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IF: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PGAM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2676 | Hi-Affi™ Recombinant Rabbit Anti-PGAM2 Monoclonal Antibody (DS2676AB) | IP, IHC-P, FC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5337 | Anti-PGAM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5338 | Anti-Mouse PGAM2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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