Human Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0504-FY) (CAT#: HPAB-0504-FY)
This product is a recombinant human antibody that recognizes P. aeruginosa OprF. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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SDS-PAGE
Figure 1 Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0504-FY) in SDS-PAGE
SDS-PAGE analysis of HPAB-0504-FY in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively.
SEC-HPLC
Figure 2 Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0504-FY) in SEC-HPLC
The purity of HPAB-0504-FY was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0504-FY) in WB
Western blot analysis of HPAB-0504-FY was performed by loading P. aeruginosa OprF protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0504-FY and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 4 Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0504-FY) in ELISA
ELISA analysis of HPAB-0504-FY was performed by coating with P. aeruginosa OprF protein (His tag). Then blocked with BSA and incubated with HPAB-0504-FY. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human OprF
- Species Reactivity
- P. aeruginosa
- Applications
- WB, ELISA
- Related Disease
- Pseudomonas aeruginosa infections
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- OprF; P. aeruginosa OprF
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0505-FY | Human Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0505-FY) | WB, ELISA | Human IgG |
| HPAB-0506-FY | Human Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0506-FY) | WB, ELISA | Human IgG |
| HPAB-0507-FY | Human Anti-P. aeruginosa OprF Recombinant Antibody (HPAB-0507-FY) | WB, ELISA | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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