Mouse Anti-PFKP Recombinant Antibody (VS3-FY2833) (CAT#: VS3-FY2833)
This anti-PFKP antibody is a mouse recombinant monoclonal antibody that has a high affinity for human, african green monkey and mouse PFKP protein. The calculated molecular weight of the protein is about 85.6 kDa. The monoclonal antibody can be used in the area of Glycogen Storage Disease Vii and Renal Cell Carcinoma.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56484/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56484/if_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic and dot like nucleolar positivity in neuronal cells and strong cytoplasmic staining in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic and dot like nucleolar positivity in neuronal cells and strong cytoplasmic staining in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43286_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43286_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_7_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_4_6.jpg
Figure 6 Skin
(Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Langerhans Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43286_B_9_1.jpg
(Keratinocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Langerhans Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43286_B_9_1.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18257/43136_A_7_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067057-PFKP
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067057-PFKP
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, African Green Monkey and Mouse PFKP
- Species Reactivity
- Human, African Green Monkey, Mouse
- Applications
- Western Blot
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 85.6 kDa.
- Related Disease
- Glycogen Storage Disease Vii and Renal Cell Carcinoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Phosphofructokinase, Platelet; Phosphofructo-1-Kinase Isozyme C; -Phosphofructokinase Type C; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-C; PFK-P
- Gene ID
- 5214
- UniProt ID
- Q01813
- Sequence Similarities
- Belongs to the phosphofructokinase type A (PFKA) family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylation at Ser-386 promotes interaction with ATG4B. (Q01813-PFKAP_HUMAN)
GlcNAcylation decreases enzyme activity. (Q01813-PFKAP_HUMAN)
Glycosylation at Ser540 (NX_Q01813 [NX_Q01813-1])
Ubiquitination at Lys15, Lys25, Lys109, Lys139, Lys289, Lys395, Lys400, Lys408, Lys459, Lys480, Lys486, Lys666, Lys688, Lys759, and Lys765 (NX_Q01813 [NX_Q01813-1])
Modification sites at PhosphoSitePlus (Q01813)
Glycosylation from GlyGen (Q01813) 3 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_006717512.1; XP_047281306.1; XP_054222059.1; XP_054222060.1
- Function
- Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3934z | Mouse Anti-PFKP Recombinant Antibody (clone 39B10) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| VS3-WK846 | Mouse Anti-PFKP Recombinant Antibody (VS3-WK846) | WB | Mouse IgG |
| VS3-FY1127 | Recombinant Rabbit Anti-PFKP Antibody (clone R05-4H9) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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