Human Anti-Phospholipid Recombinant Antibody (clone LJ1) (CAT#: FAMAB-0015WJ)

This product is a human antibody developed by fusing the peripheral blood lymphocytes of three patients with SLE with a mouse human heteromyeloma cell line. This antibody binds to a variety of anionic phospholipids, total histones but none binds to ssDNA or dsDNA.


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FC

Figure 1 LJ1 MoAb binds cells undergoing apoptosis.

Figure 1 LJ1 MoAb binds cells undergoing apoptosis.

The binding of LJ1 MoAb to U937 cells was evaluated in a cell population treated with the apoptotic stimulus. 'early' apoptotic cells with low propidium iodide staining (lower right quadrant) and 'late' apoptotic cells with higher propidium iodide staining (upper right quadrant).

Pittoni, V., Ravirajan, C. T., Donohoe, S., Machin, S. J., Lydyard, P. M., & Isenberg, D. A. (2000). Human monoclonal anti‐phospholipid antibodies selectively bind to membrane phospholipid and β2‐glycoprotein I (β2‐GPI) on apoptotic cells. Clinical & Experimental Immunology, 119(3), 533-543.

ELISA

Figure 2 Binding curves for LJ1 against CL in a standard antiphospholipid antibodies (APL) assay.

Figure 2 Binding curves for LJ1 against CL in a standard antiphospholipid antibodies (APL) assay.

CL in a modified assay, histone at 2.5 μg/ml, histone at 1.0 μg/ml.

Menon, S., Rahman, M. A. A., Ravirajan, C. T., Kandiah, D., Longhurst, C. M., McNally, T., ... & Isenberg, D. A. (1997). The production, binding characteristics and sequence analysis of four human IgG monoclonal antiphospholipid antibodies. Journal of autoimmunity, 10(1), 43-57.

ELISA

Figure 3 Inhibition of the binding of monoclonal APL to CL-coated ELISA plates by co-incubation with serial concentrations of CL.

Figure 3 Inhibition of the binding of monoclonal APL to CL-coated ELISA plates by co-incubation with serial concentrations of CL.

Inhibition of the binding of LJ1, AH2, DA3 and UK4.

Menon, S., Rahman, M. A. A., Ravirajan, C. T., Kandiah, D., Longhurst, C. M., McNally, T., ... & Isenberg, D. A. (1997). The production, binding characteristics and sequence analysis of four human IgG monoclonal antiphospholipid antibodies. Journal of autoimmunity, 10(1), 43-57.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG3, λ
  • Specificity
  • Phospholipid
  • Clone
  • LJ1
  • Applications
  • FC, ELISA
  • Related Disease
  • Systemic lupus erythematosus

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Phospholipid antibody has been reported in applications of Flow Cytometry, Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • Phospholipid

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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