Human Anti-Phospholipid Recombinant Antibody (clone LJ1)
CAT#: FAMAB-0015WJ
This product is a human antibody developed by fusing the peripheral blood lymphocytes of three patients with SLE with a mouse human heteromyeloma cell line. This antibody binds to a variety of anionic phospholipids, total histones but none binds to ssDNA or dsDNA.
Published Data
FC
Figure 1 LJ1 MoAb binds cells undergoing apoptosis.
The binding of LJ1 MoAb to U937 cells was evaluated in a cell population treated with the apoptotic stimulus. 'early' apoptotic cells with low propidium iodide staining (lower right quadrant) and 'late' apoptotic cells with higher propidium iodide staining (upper right quadrant).
Pittoni, V., Ravirajan, C. T., Donohoe, S., Machin, S. J., Lydyard, P. M., & Isenberg, D. A. (2000). Human monoclonal anti‐phospholipid antibodies selectively bind to membrane phospholipid and β2‐glycoprotein I (β2‐GPI) on apoptotic cells. Clinical & Experimental Immunology, 119(3), 533-543.
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Specifications
- Host Species
- Human
- Type
- Human IgG3, λ
- Specificity
- Phospholipid
- Clone
- LJ1
- Applications
- FC, ELISA
- Related Disease
- Systemic lupus erythematosus
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Phospholipid antibody has been reported in applications of Flow Cytometry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Phospholipid
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone LJ1"
- CAT
- Product Name
See other products for "Phospholipid"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1133-CN | Mouse Anti-Phospholipid Recombinant Antibody (HPAB-1133-CN) | WB, ELISA, IF, IHC, FC | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0015WJ-F(E) | Human Anti-Phospholipid Recombinant Antibody (clone LJ1); Fab Fragment | ELISA, IF | Human Fab |
| FAMAB-0016WJ-F(E) | Human Anti-Phospholipid Recombinant Antibody Fab Fragment (FAMAB-0016WJ-F(E)) | ELISA, IF | Human Fab |
| FAMAB-0017WJ-F(E) | Human Anti-Phospholipid Recombinant Antibody Fab Fragment (FAMAB-0017WJ-F(E)) | ELISA, IF | Human Fab |
| FAMAB-0098WJ-F(E) | Human Anti-Phospholipid Recombinant Antibody (clone STO 103); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0101WJ-F(E) | Human Anti-Phospholipid Recombinant Antibody (clone BH1); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0015WJ-S(P) | Human Anti-Phospholipid Recombinant Antibody (clone LJ1); scFv Fragment | ELISA, IF | Human scFv |
| FAMAB-0016WJ-S(P) | Human Anti-Phospholipid Recombinant Antibody scFv Fragment (FAMAB-0016WJ-S(P)) | ELISA, IF | Human scFv |
| FAMAB-0017WJ-S(P) | Human Anti-Phospholipid Recombinant Antibody scFv Fragment (FAMAB-0017WJ-S(P)) | ELISA, IF | Human scFv |
| FAMAB-0098WJ-S(P) | Human Anti-Phospholipid Recombinant Antibody (clone STO 103); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0101WJ-S(P) | Human Anti-Phospholipid Recombinant Antibody (clone BH1); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0016WJ | Human Anti-Phospholipid Recombinant Antibody (FAMAB-0016WJ) | ELISA | Human IgG1, λ |
| FAMAB-0017WJ | Human Anti-Phospholipid Recombinant Antibody (FAMAB-0017WJ) | ELISA | Human IgG3, λ |
| FAMAB-0018WJ | Human Anti-Phospholipid Recombinant Antibody (FAMAB-0018WJ) | ELISA | Human IgG1, λ |
| FAMAB-0098WJ | Human Anti-Phospholipid Recombinant Antibody (clone STO 103) | ELISA, WB | Human IgG |
| FAMAB-0101WJ | Human Anti-Phospholipid Recombinant Antibody (clone BH1) | ELISA | Human IgG |
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