Mouse Anti-phOx Recombinant Antibody (clone 12G10) (CAT#: FAMAB-0266CQ)
This product is a recombinant mouse antibody clone 12G10, which specifically binds to phOx.
Specific Inquiry
Figure 1 Non-ligand-bound β1 integrin is a retrograde cargo.
Immunolabelling assay was performed using antibodies that recognize specific conformational states of β1 integrin: 12G10 and 9EG7 antibodies against the ligand-bound state (n = 3 independent experiments for each antibody) and mAb13 antibody against the non-ligand-bound state (n=3 independent experiments). For fibronectin, a Cy3-labelled 50K fragment (FN-50K–Cy3) was used (graph represents n=3 independent experiments). Means ± s.d. Insets indicate the Golgi compartment.
Shafaq-Zadah, M., Gomes-Santos, C. S., Bardin, S., Maiuri, P., Maurin, M., Iranzo, J., ... & Johannes, L. (2016). Persistent cell migration and adhesion rely on retrograde transport of β 1 integrin. Nature cell biology, 18(1), 54.
Figure 2 Non-ligand-bound β1 integrin is a retrograde cargo.
Co-localization of different integrins and conformational states of β1 integrin with the vps35 subunit of retromer (means ± s.d.; a representative of 2 independent experiments is shown). The RGB profiler plugin from ImageJ was used to plot co-localization. Quantifications show Manders' co-localization coefficient.
Shafaq-Zadah, M., Gomes-Santos, C. S., Bardin, S., Maiuri, P., Maurin, M., Iranzo, J., ... & Johannes, L. (2016). Persistent cell migration and adhesion rely on retrograde transport of β 1 integrin. Nature cell biology, 18(1), 54.
IP
Figure 3 Non-ligand-bound β1 integrin is a retrograde cargo.
Immunoprecipitation assay was performed using antibodies that recognize specific conformational states of β1 integrin: 12G10 and 9EG7 antibodies against the ligand-bound state (n = 3 independent experiments for each antibody) and mAb13 antibody against the non-ligand-bound state (n=3 independent experiments). For fibronectin, a Cy3-labelled 50K fragment (FN-50K–Cy3) was used (graph represents n=3 independent experiments). Means ± s.d. Insets indicate the Golgi compartment.
Shafaq-Zadah, M., Gomes-Santos, C. S., Bardin, S., Maiuri, P., Maurin, M., Iranzo, J., ... & Johannes, L. (2016). Persistent cell migration and adhesion rely on retrograde transport of β 1 integrin. Nature cell biology, 18(1), 54.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- phenyl-oxazolone (phOx)
- Clone
- 12G10
- Applications
- ELISA, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunoprecipitation.
Target
- Alternative Names
- phOx; phenyl-oxazolone
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 12G10"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-002 | Mouse Anti-phOx Recombinant Antibody (clone NQ10-1.12) | WB, ELISA | Mouse IgG |
| PABC-003 | Mouse Anti-phOx Recombinant Antibody (clone NQ16-113.8) | WB, IF, FuncS | Mouse IgG |
| FAMAB-0246YC | Mouse Anti-phOx Recombinant Antibody (clone 19E11) | ELISA | Mouse IgM |
| FAMAB-0247YC | Mouse Anti-phOx Recombinant Antibody (clone 17G5) | ELISA | Mouse IgG1 |
| FAMAB-0106-CN | Human Anti-phOx Recombinant Antibody (clone αphOx-15) | ELISA | Human IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0246-YC-S(P) | Mouse Anti-phOx Recombinant Antibody (clone 19E11); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0247-YC-S(P) | Mouse Anti-phOx Recombinant Antibody (clone 17G5); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0038WJ-S(P) | Mouse Anti-phOx Recombinant Antibody (clone 4D6); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0041WJ-S(P) | Mouse Anti-phOx Recombinant Antibody (clone 19G7); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0042WJ-S(P) | Mouse Anti-phOx Recombinant Antibody (clone 9D8); scFv Fragment | ELISA, WB | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0246-YC-F(E) | Mouse Anti-phOx Recombinant Antibody (clone 19E11); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0247-YC-F(E) | Mouse Anti-phOx Recombinant Antibody (clone 17G5); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0264CQ-F(E) | Mouse Anti-phOx Recombinant Antibody (clone 18C10); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0265CQ-F(E) | Mouse Anti-phOx Recombinant Antibody (clone 6F6); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0266CQ-F(E) | Mouse Anti-phOx Recombinant Antibody (clone 12G10); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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