Mouse Anti-RAB11FIP1 Recombinant Antibody (clone 1A12B9D8) (CAT#: VS7-0425-WR732)
This product is a mouse IgG1 antibody that binds to human RAB11FIP1. The antibody 1A12B9D8 recognizes specific amino acid domain of human RAB11FIP1 (AA: 130-271). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23904/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23904/if_selected.jpg
Figure 2 IF staining of human cell line K-562
(Immunofluorescent staining of human cell line K-562 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/1854_G11_2_selected.jpg
(Immunofluorescent staining of human cell line K-562 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/1854_G11_2_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/53167_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Microvilli Staining: High Intensity: Strong Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/53167_A_9_3.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/53167_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24010/53167_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156675-RAB11FIP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156675-RAB11FIP1
Specifications
- Immunogen
- Recombinant human RAB11FIP1 (AA: 130-271) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAB11FIP1
- Species Reactivity
- Human, Mouse
- Clone
- 1A12B9D8
- Molecule Class
- Rab11 Family Interacting Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAB11 Family Interacting Protein 1; RAB11 Family Interacting Protein 1 (Class I); Rab11-FIP1; RCP; Rab11 Family-Interacting Protein 1; Rab-Interacting Recycling Protein; RAB11 Coupling Protein
- Gene ID
- 80223
- UniProt ID
- Q6WKZ4
- Long Name
- RAB11 Family Interacting Protein 1
- Cellular Localization
- Cytoplasmic vesicle; Endosome; Membrane
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001002814.2
- Function
- A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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