Mouse Anti-RAD9A Recombinant Antibody (VS3-FY2859) (CAT#: VS3-FY2859)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat, Monkey RAD9A

• Species Reactivity
• Human, Mouse, Rat, Monkey

• Applications
• Western Blot, Immunoprecipitation

• Conjugate
• Unconjugated, FITC

• Related Disease
• Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Xeroderma Pigmentosum, Variant Type

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunoprecipitation: 1/20
  Western Blot: 1/500-1/1000

• Alternative Names
• RAD9 Checkpoint Clamp Component A; DNA Repair Exonuclease Rad9 Homolog A; HRAD9; Cell Cycle Checkpoint Control Protein RAD9A; RAD9 Homolog A (S. Pombe); RAD9 (S. Pombe) Homolog; RAD9 Homolog A; EC 3.1.11.2; RAD9

• Gene ID
• 5883

• UniProt ID
• Q99638

• Sequence Similarities
• Belongs to the rad9 family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage (PubMed:12628935).
  Hyperphosphorylated by PRKCD and ABL1 upon DNA damage (PubMed:11971963, 12709442).
  Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex (PubMed:12628935).
  Phosphorylated at Ser-341 and Ser-387 by CK2, promoting interaction with TOPBP1 (PubMed:17575048, 20545769).
  (Q99638-RAD9A_HUMAN)
  Modification sites at PhosphoSitePlus (Q99638)
  Modification sites at neXtProt (NX_Q99638)
  Glycosylation from GlyGen (Q99638) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_016873587.1; XP_047283337.1; XP_047283338.1; XP_047283339.1

• Function
• Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:21659603).
  The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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