Mouse Anti-RAD9A Recombinant Antibody (VS3-FY2859) (CAT#: VS3-FY2859)
The anti-RAD9A antibody is a mouse recombinant monoclonal antibody that has been specifically designed to target the human RAD9A protein. It cross-reacts with mouse, rat, monkey species. This monoclonal antibody is suitable for employment in Immunoprecipitation (1/20), Western Blot (1/500-1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/19_G11_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/19_G11_1_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48155/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48155/if_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79979/1999_F9_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79979/1999_F9_3_selected.jpg
Figure 4 Pancreas
(High expression in pancreas)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_1_3_val_selected.jpg
(High expression in pancreas)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_1_3_val_selected.jpg
Figure 5 Skin
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_B_9_1.jpg
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_B_9_1.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_8_8.jpg
Figure 7 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_7_3.jpg
Figure 8 Kidney
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_8_5.jpg
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6725/18520_A_8_5.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172613-RAD9A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172613-RAD9A
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat, Monkey RAD9A
- Species Reactivity
- Human, Mouse, Rat, Monkey
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Xeroderma Pigmentosum, Variant Type
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Target
- Alternative Names
- RAD9 Checkpoint Clamp Component A; DNA Repair Exonuclease Rad9 Homolog A; HRAD9; Cell Cycle Checkpoint Control Protein RAD9A; RAD9 Homolog A (S. Pombe); RAD9 (S. Pombe) Homolog; RAD9 Homolog A; EC 3.1.11.2; RAD9
- Gene ID
- 5883
- UniProt ID
- Q99638
- Sequence Similarities
- Belongs to the rad9 family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage (PubMed:12628935).
Hyperphosphorylated by PRKCD and ABL1 upon DNA damage (PubMed:11971963, 12709442).
Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex (PubMed:12628935).
Phosphorylated at Ser-341 and Ser-387 by CK2, promoting interaction with TOPBP1 (PubMed:17575048, 20545769).
(Q99638-RAD9A_HUMAN)
Modification sites at PhosphoSitePlus (Q99638)
Modification sites at neXtProt (NX_Q99638)
Glycosylation from GlyGen (Q99638) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016873587.1; XP_047283337.1; XP_047283338.1; XP_047283339.1
- Function
- Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:21659603).
The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1764CT | Recombinant Mouse anti-Human RAD9A Monoclonal antibody (04B646) | ICC/IF, IP, WB | |
| VS3-QX954 | Mouse Anti-RAD9A Recombinant Antibody (clone 3A3-A7-F8) | WB | Mouse IgG2b |
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