PTM™ Rabbit Anti-RB1 (pThr356) Recombinant Antibody (VS13-YC985) (CAT#: VS13-YC985)

• Immunogen
• Synthetic phosphopeptide of human Rb (pThr356).

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• The phosphorylated RB1 (Thr356) of human and rat.

• Species Reactivity
• Human, Rat

• Applications
• WB, IHC-P, IP

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Affinity Chromatography purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.

• Preservative
• 0.01% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunohistochemistry (IHC): 1/50-1/100
  Immunoprecipitation (IP): 1/20

• Alternative Names
• RB Transcriptional Corepressor 1; Protein Phosphatase 1, Regulatory Subunit 130; Prepro-Retinoblastoma-Associated Protein; Retinoblastoma 1; P105-Rb; Pp110; PRb; RB;

• Gene ID
• 5925

• UniProt ID
• P06400

• Long Name
• RB Transcriptional Corepressor 1

• Sequence Similarities
• Belongs to the retinoblastoma protein (RB) family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated

• Protein Refseq
• NP_000312.2

• Function
• Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802).
  The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802).
  Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802).
  Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802).
  RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261).
  Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).
  (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody