Mouse Anti-Rheumatoid factor Recombinant Antibody (clone 6-19) (CAT#: FAMAB-0289CQ)

Recombinant Mouse Antibody clone 6-19, which is specific to Rheumatoid factor. 6-19 is an anti-IgG2a rheumatoid factor antibody, derived from lupus-prone MRL-Faslpr mice.


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IF

Figure 1 Representative glomerular appearance and immune deposits in BALB/c mice implanted with 46-42 IgA RF hybridoma.

Figure 1 Representative glomerular appearance and immune deposits in BALB/c mice implanted with 46-42 IgA RF hybridoma.

Only minimal extent of IgA, IgG2a, and C3 deposits in glomeruli.

Otani, M., Nakata, J., Kihara, M., Leroy, V., Moll, S., Wada, Y., & Izui, S. (2012). O-glycosylated IgA rheumatoid factor induces IgA deposits and glomerulonephritis. Journal of the American Society of Nephrology: JASN, 23(3), 438–446.

ELISA

Figure 2 Elution profiles of 6-19 and 46-42 IgA RF mAbs on Sephadex G200 gel filtration column.

Figure 2 Elution profiles of 6-19 and 46-42 IgA RF mAbs on Sephadex G200 gel filtration column.

Relative concentrations of IgA (open circle) and IgA anti-IgG2a RF (closed circle) in each fraction, expressed as OD values at 405 nm, were estimated by ELISA.

Otani, M., Nakata, J., Kihara, M., Leroy, V., Moll, S., Wada, Y., & Izui, S. (2012). O-glycosylated IgA rheumatoid factor induces IgA deposits and glomerulonephritis. Journal of the American Society of Nephrology: JASN, 23(3), 438–446.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG3
  • Specificity
  • Rheumatoid factor
  • Species Reactivity
  • Mouse
  • Clone
  • 6-19
  • Applications
  • FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Rheumatoid Factor; RF

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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