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Rabbit Anti-RNF8 Recombinant Antibody (clone R06-6O7) (CAT#: VS3-XY3488)

This is a recombinant rabbit antibody targeting the human RNF8 (Ring finger protein 8). The antibody specifically reacts with human and rat RNF8. It has been reported for use in Western Blot. The recommended dilutions of Western blot is 1/500-1/1000.


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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64925/1154_G3_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong nucleolar and cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_B_8_5.jpg
Figure 4 Colon
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_7_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_9_5.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-698)
Figure 7 RNA cell line category: Cell line enhanced (U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112130-RNF8

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat RNF8
  • Species Reactivity
  • Human, Rat
  • Clone
  • R06-6O7
  • Applications
  • WB
  • Related Disease
  • Riddle Syndrome; Dyskeratosis Congenita, Autosomal Recessive 3

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000

Target

  • Alternative Names
  • hRNF8
  • Long Name
  • Ring finger protein 8
  • Cellular Localization
  • Nucleus, Cytoplasm
  • Post Translation Modifications
  • Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin.
    'Lys-63' polyubiquitination is mediated by UBE2N.
    'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
    (O76064-RNF8_HUMAN)
    Modification sites at PhosphoSitePlus (O76064)
    Modification sites at neXtProt (NX_O76064)
  • Function
  • E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.
    Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF).
    Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RNF8"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3090 Hi-Affi™ Recombinant Rabbit Anti-RNF8 Monoclonal Antibody (DS3090AB) WB IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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