Rabbit Anti-RNF8 Recombinant Antibody (clone R06-6O7)
CAT#: VS3-XY3488
This is a recombinant rabbit antibody targeting the human RNF8 (Ring finger protein 8). The antibody specifically reacts with human and rat RNF8. It has been reported for use in Western Blot. The recommended dilutions of Western blot is 1/500-1/1000.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64925/1154_G3_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nucleolar and cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50731/116346_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112130-RNF8
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat RNF8
- Species Reactivity
- Human, Rat
- Clone
- R06-6O7
- Applications
- WB
- Related Disease
- Riddle Syndrome; Dyskeratosis Congenita, Autosomal Recessive 3
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- hRNF8
- Gene ID
- 9025
- UniProt ID
- O76064
- Long Name
- Ring finger protein 8
- Cellular Localization
- Nucleus, Cytoplasm
- Post Translation Modifications
- Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin.
'Lys-63' polyubiquitination is mediated by UBE2N.
'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
(O76064-RNF8_HUMAN)
Modification sites at PhosphoSitePlus (O76064)
Modification sites at neXtProt (NX_O76064)
- Protein Refseq
- NP_898901.1; NP_003949.1
- Function
- E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.
Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF).
Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Name
Recommended Dilution Buffer
- CAT
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Secondary Antibody
- CAT
- Product Name
See other products for "RNF8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3090 | Hi-Affi™ Recombinant Rabbit Anti-RNF8 Monoclonal Antibody (DS3090AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6166 | Anti-RNF8 Immunohistochemistry Kit | IHC |
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