Mouse Anti-RTB Recombinant Antibody (clone RBC11) (CAT#: FAMAB-0295CQ)
Recombinant Mouse Antibody clone RBC11, which is specific to Ricin B chain. This antibody recognizes Ricinus communis ricin toxin B chain.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 In vitro neutralization of ricin by polyclonal horse Fab preparations and MAbs.
Panel A. Agilent microcapillary electrophoresis of intact monoclonal RTAs, MAb against RTA (RAC18 IgG vs. RAC18 Fab), and the polyclonal horse anti-ricin "Fab" preparation, under reducing and non-reducing conditions. The horse antibody preparation consists primarily of F(ab)'2 fragments. Panel B. ELISA binding of antibodies to plates coated with RT. Different secondary enzyme-conjugated antibodies were used to detect the horse and mouse Igs. Antibody concentration represents total protein. For MAbs, 100% is RT-specific antibody, whereas for the horse Fab, only 3.2% is RT specific. Panel C. In vitro neutralization of RT cytotoxicity by intact antibody and Fab fragments. Antibody concentration represents total protein. No antibody and control antibodies yield identical curves. Panel D. In vitro neutralization by high titer antibodies against RTA, (RAC), RTB (RBC), or both. Antibodies were used at 10 µg/mL.
Pincus, S. H., Smallshaw, J. E., Song, K., Berry, J., & Vitetta, E. S. (2011). Passive and active vaccination strategies to prevent ricin poisoning. Toxins, 3(9), 1163-1184.
Specifications
- Immunogen
- Ricin B chain
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Ricinus communis ricin toxin B chain
- Species Reactivity
- Ricinus communis
- Clone
- RBC11
- Applications
- ELISA, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Ricin B chain; Ricin B lectin; Ricin chain B; Ricin precursor
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single Domain Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-030 | Recombinant Anti-RTB VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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