Recombinant Mouse Anti-SEK Antibody (2C2) (CAT#: FAMAB-0300CQ)
This product is a recombinant mouse antibody clone 2C2, which specifically binds to Staphylococcal enterotoxin K.
Specific Inquiry
ELISA
Figure 1 Direct ELISA showing binding of anti-SEs recombinant antibodies with SEA-His antigen.
Antibodies expression in the cell lysate transfected with plasmid p2C2HILO or p5C12HILO was much more higher than that of the pcDNA3.1(+)-transfected cell lysate and a continually increased expression product after 12 days posttransfection (PT) and tended to be constant later.
He, X., Zhang, L., Liu, P., Liu, L., Deng, H., & Huang, J. (2015). Construction and characterization of VL-VH tail-parallel genetically engineered antibodies against staphylococcal enterotoxins. Immunologic research, 61(3), 281–293.
FC
Figure 2 Detection recombinant antibodies expressed in BHK21 cells by FCM.
2C2 recombinant antibodies had the similar ability of binding to different serotype of SEs, including natural SEA and other SEs-His recombinant antigens.
He, X., Zhang, L., Liu, P., Liu, L., Deng, H., & Huang, J. (2015). Construction and characterization of VL-VH tail-parallel genetically engineered antibodies against staphylococcal enterotoxins. Immunologic research, 61(3), 281–294.
IF
Figure 3 Detection of recombinant antibodies expressed in BHK21 cells by IFA.
The cells transfected with p2C2HILO individually bond to 10 μg/mL natural SEA, SEA-His, SEG-His, SEK-His, SEO-His, SEQ-His, and SEU-His.
He, X., Zhang, L., Liu, P., Liu, L., Deng, H., & Huang, J. (2015). Construction and characterization of VL-VH tail-parallel genetically engineered antibodies against staphylococcal enterotoxins. Immunologic research, 61(3), 281–295.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Staphylococcal enterotoxin K
- Species Reactivity
- S. aureus
- Clone
- 2C2
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay
- Related Disease
- S. aureus infections
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- SEK; Staphylococcal enterotoxin K
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0300CQ-F(E) | Recombinant Mouse Anti-SEK Antibody Fab Fragment (2C2) | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0300CQ-S(P) | Recombinant Mouse Anti-SEK Antibody scFv Fragment (2C2) | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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