Rabbit Anti-SNRPD3 Recombinant Antibody (clone R09-8I7)
CAT#: VS3-FY1883
Clone R09-8I7 is a rabbit recombinant antibody that exhibits high affinity to human SNRPD3 protein, a calculated weight of 14 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-8I7 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/967_G9_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100028-SNRPD3
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Specifications
- Immunogen
- Synthetic peptide of human SNRPD3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat SNRPD3
- Species Reactivity
- Human, Rat
- Clone
- R09-8I7
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 14 kDa, while the observed molecular weight stands at 14 kDa.
- Related Disease
- Lupus Erythematosus and Spinal Muscular Atrophy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Small Nuclear Ribonucleoprotein D3 Polypeptide; Small Nuclear Ribonucleoprotein D3 Polypeptide 18kDa; SnRNP Core Protein D3; Sm-D3; Small Nuclear Ribonucleoprotein D3 Polypeptide (18kD); Small Nuclear Ribonucleoprotein Sm D3; SMD3
- Gene ID
- 6634
- UniProt ID
- P62318
- Sequence Similarities
- Belongs to the snRNP core protein family.
- Cellular Localization
- Cytoplasm, Nucleus, Spliceosome
- Post Translation Modifications
- Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs. (P62318-SMD3_HUMAN)
Ubiquitination at Lys8 and Lys78 (NX_P62318 [NX_P62318-1])
Modification sites at PhosphoSitePlus (P62318)
Glycosylation from GlyGen (P62318) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001265585.1; NP_004166.1
- Function
- Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SNRPD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1535z | Mouse Anti-SNRPD3 Recombinant Antibody (clone 26C1) | WB, Dot | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1703 | PE-B*57:01/Human SNRPD3 (RVAQLEQVYI) MHC Tetramer | FCM | |
| MHC-LC1704 | APC-B*57:01/Human SNRPD3 (RVAQLEQVYI) MHC Tetramer | FCM | |
| MHC-LC1705 | BV421-B*57:01/Human SNRPD3 (RVAQLEQVYI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2135 | Anti-SNRPD3 Immunohistochemistry Kit | IHC |
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