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    Rabbit Anti-SNRPD3 Recombinant Antibody (clone R09-8I7) (CAT#: VS3-FY1883)

    Clone R09-8I7 is a rabbit recombinant antibody that exhibits high affinity to human SNRPD3 protein, a calculated weight of 14 kDa. It cross-reacts with rat.
    The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-8I7 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/967_G9_2_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human small intestine
    Figure 2 IHC staining of human small intestine
    (Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_9_3.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_7_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_4_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1170/3542_A_8_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100028-SNRPD3

    Specifications

    • Immunogen
    • Synthetic peptide of human SNRPD3
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Rat SNRPD3
    • Species Reactivity
    • Human, Rat
    • Clone
    • R09-8I7
    • Applications
    • Western Blot
    • MW
    • The calculated weight of the target protein is approximately 14 kDa, while the observed molecular weight stands at 14 kDa.
    • Related Disease
    • Lupus Erythematosus and Spinal Muscular Atrophy

    Product Property

    • Purification
    • Affinity Purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000

    Target

    • Alternative Names
    • Small Nuclear Ribonucleoprotein D3 Polypeptide; Small Nuclear Ribonucleoprotein D3 Polypeptide 18kDa; SnRNP Core Protein D3; Sm-D3; Small Nuclear Ribonucleoprotein D3 Polypeptide (18kD); Small Nuclear Ribonucleoprotein Sm D3; SMD3
    • Sequence Similarities
    • Belongs to the snRNP core protein family.
    • Cellular Localization
    • Cytoplasm, Nucleus, Spliceosome
    • Post Translation Modifications
    • Methylated on arginine residues by PRMT5 and PRMT7; probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs. (P62318-SMD3_HUMAN)
      Ubiquitination at Lys8 and Lys78 (NX_P62318 [NX_P62318-1])
      Modification sites at PhosphoSitePlus (P62318)
      Glycosylation from GlyGen (P62318) 1 site, 1 O-linked glycan (1 site)
    • Function
    • Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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