Rabbit Anti-TFE3 Recombinant Antibody (clone R09-3G6)
CAT#: VS3-FY1900
Clone R09-3G6 is a rabbit IgG recombinant monoclonal antibody that is specifically engineered to target human TFE3 protein, a calculated weight of 62 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-3G6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23881/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23881/53078_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23881/53078_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23881/53078_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23881/53078_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000068323-TFE3
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Specifications
- Immunogen
- Recombinant human TFE3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse TFE3
- Species Reactivity
- Human, Mouse
- Clone
- R09-3G6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 62 kDa, while the observed molecular weight stands at 62 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Transcription Factor Binding To IGHM Enhancer 3; Class E Basic Helix-Loop-Helix Protein 33; Transcription Factor E Family, Member A; BHLHe33; Transcription Factor For Immunoglobulin Heavy-Chain Enhancer 3; Transcription Factor For IgH Enhancer
- Gene ID
- 7030
- UniProt ID
- P19532
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Sumoylated; does not affect dimerization with MITF. (P19532-TFE3_HUMAN)
Phosphorylation ar Ser-47 and Ser-321 by MTOR via non-canonical mTORC1 pathway regulates its stability and subcellular location, respectively (PubMed:21209915, 24448649, 30733432, 36608670).
When nutrients are present, phosphorylation by MTOR at Ser-47 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation (PubMed:36608670).
When nutrients are present, phosphorylation by MTOR at Ser-321 also promotes association with 14-3-3/YWHA adapters and retention in the cytosol (PubMed:24448649, 30733432).
Phosphorylation at Ser-47 plays a more critical role than phosphorylation at Ser-321 for TFE3 inactivation (PubMed:36608670).
Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and transcription factor activity (PubMed:30733432, 36608670).
- Protein Refseq
- XP_024308200.1; XP_054183633.1; XP_054189387.1; NP_001269071.1; NP_006512.2
- Function
- Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity).
In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4⁺ T-cells and thymus-dependent humoral immunity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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