Mouse Anti-WNV E Recombinant Antibody (clone E-85) (CAT#: FAMAB-0737WJ)

Figure 1 Binding of scFvs to rWNV-E as measured by ELISA.

ELISA plates were coated with rWNV-E (100 ng/well) overnight and incubated with serial dilutions of antibodies. Anti-His-HRP (1:4,000) was used as a secondary antibody, and the plates were developed and read at 450 nm (OD450).

Kanai, R., Kar, K., Anthony, K., Gould, L. H., Ledizet, M., Fikrig, E., ... & Modis, Y. (2006). Crystal structure of West Nile virus envelope glycoprotein reveals viral surface epitopes. Journal of virology, 80(22), 11000-11008.

Figure 2 Binding of scFvs to rWNV-E.

rWNV-E (10 μg/gel) was run on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel under reducing (in the presence of 2-mercaptoethanol) or nonreducing conditions and blotted to nitrocellulose. Blots were incubated with scFvs (1 μg/ml), and binding was detected by incubation with anti-His-HRP (1:4,000). Lane Ab refers to control for background anti-His-HRP antibody binding to rWNV-E.

Kanai, R., Kar, K., Anthony, K., Gould, L. H., Ledizet, M., Fikrig, E., ... & Modis, Y. (2006). Crystal structure of West Nile virus envelope glycoprotein reveals viral surface epitopes. Journal of virology, 80(22), 11000-11008.

Figure 3 Binding of scFv-Fcs to WNV E protein ectodomain, DI/DII, and DIII.

The binding of scFv-Fcs to yeast displaying either the E protein ectodomain, DI/DII, or DII was measured by fluorescence-activated cell sorting. Yeast cells displaying the indicated fusions were incubated with scFv-Fcs, followed by incubation with the appropriate secondary antibody conjugated to Alexa Fluor 647, and the binding was measured by flow cytometry. The percentage of cells binding to each region was determined after subtracting background binding to control yeast cells expressing only the pYD1 vector. Mouse MAbs E24 and E53 served as positive controls for binding to DIII and DI/DII, respectively.

Kanai, R., Kar, K., Anthony, K., Gould, L. H., Ledizet, M., Fikrig, E., ... & Modis, Y. (2006). Crystal structure of West Nile virus envelope glycoprotein reveals viral surface epitopes. Journal of virology, 80(22), 11000-11008.