GeneSpark™ Human ATF-3 Exon 1 Primers (VS-0725-FY53)
CAT#: VS-0725-FY53
The Human ATF-3 Exon 1 Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting exon 1 of the human ATF-3 (Activating Transcription Factor 3) gene. ATF-3 is a stress-inducible transcription factor involved in a wide range of cellular responses, including apoptosis, cell cycle arrest, and differentiation. Its rapid induction upon stress makes its gene locus an excellent target for studying dynamic chromatin changes. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers in ChIP assays are highly specific probes. Their main function is to detect and quantify particular DNA sequences that have been isolated via immunoprecipitation, ultimately revealing crucial protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
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