GeneSpark™ Human C/EBPδ Promoter Primers (VS-0725-FY91)
CAT#: VS-0725-FY91
The Human C/EBPδ Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human C/EBPδ (CCAAT/Enhancer Binding Protein Delta) gene. C/EBPδ is a transcription factor involved in regulating genes related to inflammation, metabolism, and cell proliferation, often induced in response to stress. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers serve a fundamental role in ChIP assays by acting as highly accurate probes. They are essential for precisely identifying and measuring the specific DNA sequences that have been concentrated via immunoprecipitation, ultimately unveiling the intricate details of protein-DNA binding events.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
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