GeneSpark™ Human c-Fos Upstream Primers (VS-0725-FY96)
CAT#: VS-0725-FY96
The Human c-Fos Upstream Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target an upstream region of the human c-Fos gene. c-Fos is an immediate-early gene, rapidly induced by a wide array of extracellular stimuli, forming part of the AP-1 transcription factor complex. Targeting upstream regions can reveal insights into enhancer elements or proximal regulatory sequences involved in rapid transcriptional responses. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers play an indispensable role in ChIP assays by enabling the accurate identification and quantification of specific DNA sequences. Their use, after DNA enrichment via immunoprecipitation, allows researchers to precisely delineate protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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