GeneSpark™ Human CTGF Promoter Primers (VS-0725-FY34)
CAT#: VS-0725-FY34
The Human CTGF Promoter Primers are designed for amplifying DNA isolated through chromatin immunoprecipitation (ChIP) assays. This product includes optimized forward and reverse PCR primers specific to the promoter region of the human CTGF (Connective Tissue Growth Factor) gene. CTGF plays a significant role in extracellular matrix production, wound healing, and fibrosis, often implicated in various fibrotic diseases and cancers. These primers are optimized for SYBR® Green quantitative real-time PCR and are thoroughly validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They enable precise investigation of epigenetic modifications and transcription factor binding at the CTGF promoter.
Specifications
- Application
- ChIP
- Application Notes
- The core function of primers in ChIP experiments is to serve as specific probes for detecting and quantifying particular DNA fragments enriched after immunoprecipitation, thereby revealing protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Connective Tissue Growth Factor
- Introduction
- CTGF (Connective Tissue Growth Factor) is a Protein Coding gene. Diseases associated with CTGF include Systemic Scleroderma and Renal Fibrosis. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and PAK Pathway. Gene Ontology (GO) annotations related to this gene include growth factor activity and integrin binding. An important paralog of this gene is NOV.
- Alternative Names
- Connective Tissue Growth Factor; Hypertrophic Chondrocyte-Specific Protein 24; Insulin-Like Growth Factor-Binding Protein 8; IGF-Binding Protein 8; CCN Family Member 2; IGFBP-8
- Gene ID
- 1490
- UniProt ID
- P29279
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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