GeneSpark™ Human EGR1 Promoter Primers (VS-0725-FY86)
CAT#: VS-0725-FY86
The Human EGR1 Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human EGR1 (Early Growth Response 1) gene. EGR1 is an immediate-early gene, rapidly induced by diverse stimuli including growth factors, stress, and differentiation signals, playing roles in cell proliferation, differentiation, and apoptosis. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- In qPCR, the primers enable the exponential copying of the target DNA, allowing for its precise quantification. The amount of amplified DNA is directly proportional to the initial amount of target DNA present in the immunoprecipitated sample. This quantitative measure is incredibly valuable, as it not only tells us if a protein binds to a specific DNA region, but also how strongly or how frequently that binding occurs under different conditions.
For ChIP-seq, primers are used to generate libraries of DNA fragments that are then sequenced. This provides an even broader, genome-wide view of protein-DNA binding sites, and primers are essential for preparing these fragments for the sequencing process.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Early Growth Response 1
- Introduction
- The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.
- Alternative Names
- Early Growth Response 1; Nerve Growth Factor-Induced Protein A; Transcription Factor ETR103; Zinc Finger Protein 225; Early Growth Response Protein 1; Transcription Factor Zif268; Zinc Finger Protein Krox-24; NGFI-A; ZNF225
- Gene ID
- 1958
- UniProt ID
- P18146
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