GeneSpark™ Human HES5 Upstream Primers (VS-0725-FY58)
CAT#: VS-0725-FY58
The Human HES5 Upstream Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target an upstream region of the human HES5 (Hes Family BHLH Transcription Factor 5) gene. HES5 is a basic helix-loop-helix transcription factor that is a target of Notch signaling, critically involved in neural development and cell fate decisions. Targeting upstream regions can reveal insights into enhancer elements or proximal regulatory sequences. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- The primary role of primers in chromatin immunoprecipitation (ChIP) assays is to act as accurate detectors. They enable the identification and measurement of specific DNA sequences that have been concentrated through immunoprecipitation, thereby illuminating protein-DNA binding events.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Hes Family BHLH Transcription Factor 5
- Introduction
- This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer.
- Alternative Names
- Hes Family BHLH Transcription Factor 5; Class B Basic Helix-Loop-Helix Protein 38; Hairy And Enhancer Of Split 5; BHLHb38; Hairy And Enhancer Of Split 5 (Drosophila); Transcription Factor HES-5
- Gene ID
- 388585
- UniProt ID
- Q5TA89
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