GeneSpark™ Human HMOX1 Promoter Primers (VS-0725-FY101)
CAT#: VS-0725-FY101
The Human HMOX1 Promoter Primers provide a specific and optimized solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human HMOX1 (Heme Oxygenase 1) gene. HMOX1 is a stress-inducible enzyme involved in heme catabolism and acts as a potent antioxidant and anti-inflammatory agent. Its promoter is highly responsive to oxidative stress and other stimuli. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- For precisely mapping protein-DNA interactions in ChIP assays, primers are crucial. They facilitate the accurate identification and quantification of the specific DNA sequences that are enriched during the immunoprecipitation process.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Heme Oxygenase 1
- Introduction
- Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
- Alternative Names
- Heme Oxygenase 1; Heme Oxygenase (Decycling) 1; HO-1; Heat Shock Protein, 32-KD; EC 1.14.14.18; EC 1.14.99.3
- Gene ID
- 3162
- UniProt ID
- P09601
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