GeneSpark™ Human HoxA9 Promoter Primers (VS-0725-FY100)
CAT#: VS-0725-FY100
The Human HoxA9 Promoter Primers offer a highly specific solution for amplifying DNA obtained through chromatin immunoprecipitation (ChIP). This product contains a pre-mixed set of forward and reverse PCR primers designed to target the promoter region of the human HoxA9 gene. HoxA9 is a homeobox gene critical for hematopoietic stem cell self-renewal and lineage specification, and its deregulation is frequently observed in leukemia. Its precise transcriptional regulation is vital for normal hematopoiesis. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies.
Specifications
- Application
- ChIP
- Application Notes
- Primers are indispensable in ChIP assays for effectively mapping protein-DNA interactions. They enable the accurate identification and quantification of specific DNA sequences enriched during immunoprecipitation.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Human
- Storage
- Store at -20°C.
Target
- Full Name
- Homeobox A9
- Introduction
- In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.
- Alternative Names
- Homeobox A9; Homeobox Protein Hox-1G; HOX1G; Homeodomain Protein HOXA9; Homeobox Protein Hox-A9; Homeo Box A9; HOX1.7; ABD-B; HOX1
- Gene ID
- 3205
- UniProt ID
- P31269
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